期刊文献+

不同病变型J亚群禽白血病病毒LTR启动子序列分析及活性比较 被引量:7

Sequence and Promoter Efficacy Analysis of Avian leukosis Virus Subgroup J Strains of Different Pathotypes
原文传递
导出
摘要 从ALV-J中国地方分离株SCAU-HN06株(血管瘤病变型)、NX0101株和JS-nt株(骨髓瘤病变型)病毒的细胞培养物提取前病毒DNA,通过PCR扩增各毒株的LTR并克隆,随后进行测序分析。与国内外ALV-J参考毒株LTR序列比较发现:国内地方分离株与英国ALV-J原型株HPRS-103和美国ALV-J原型株ADOL-7501的LTR核苷酸序列相似性为88.0%~97.2%;LTR中的U5区及R区具有较高的保守性,而U3区内存在较大差异。将不同病变型ALV-J的LTR片段分别插入pCAT-basic载体CAT报告基因5'端。用所得的重组报告基因表达质粒转染DF-1细胞,48h后通过测定转染细胞中的CAT表达量来评价LTR启动子的活性。结果表明,SCAU-HN06株与骨髓瘤病变型ALV-J(JS-nt株,NX0101株)LTR启动子活性差异不显著。 To characterize the long terminal repeat(LTR) of the ALV-J strain which can induce hemangioma,fragments of provirus LTR of the three different ALV-J strains SCAU-HN06,NX0101 and JS-nt were amplified with a pair of specific primers,then cloned and subjected to sequence analysis.In comparison with the prototype ALV-J strains HPRS-103 and ADOL-7501,the LTRs of domestic strains(SCAU-HN06,NX0101,JS-nt and SD07lk1) had an 88.0%-97.2% nucleotide sequence identity;the U5 and R regions in the LTR had a high nucleotide similarity,while the U3 region in the LTR showed significant variance.The LTR fragments from the different ALV-J strains were inserted into the upstream of bacterial CAT gene of the plasmid pCAT-Basic,respectively.The resultant recombinant plasmids were transfected into DF-1 cells.The transfected cells were harvested 48 h post-transfection,and cell lysates were prepared for CAT expression detection.The CAT assay was performed using CAT-ELISA.The results showed that the promoter activity of the LTRSCAU-HN06 was a little higher than those of LTRJS-nt and LTRNX0101,but there was no significant difference in the promoter activity among the compared LTRs.
出处 《病毒学报》 CAS CSCD 北大核心 2010年第5期402-406,共5页 Chinese Journal of Virology
基金 国家自然科学基金(30771612) NSFC-广东联合基金(U0831002) 广东省自然科学基金(8151064201000065) 广东省科技计划项目(2009A020101006) 肉鸡产业技术体系项目(nycytx-42-G3-03)
关键词 J亚群禽白血病病毒 血管瘤 长末端重复序列 启动子活性 avian leukosis virus subgroup J hemangioma long terminal repeat promoting efficacy
  • 相关文献

参考文献5

二级参考文献22

  • 1秦爱建.J亚群白血病病毒囊膜蛋白env基因的生物学和生物化学特性[M].扬州:扬州大学,1999..
  • 2辛朝安.禽病学(第二版)[M].北京:中国农业出版社,2003..
  • 3Payne L N, Brown S R, Bunstead N, et al. A novel subgroup of exogenous avian leukosis virus in chickens. Journal of General Virology, 1991,72: 801 - 807.
  • 4Payne L N, Howes K, Gillespie A M. Host range of Rous sarcoma virus pseudotype RSV(HPRS-103) in 12 avian species:support for a new avian retrovirus envelope subgroup, designated J. Journal of General Virology ,1992, 73, 2995 - 2997.
  • 5Gharaibeh S, Brown T, Stedman N, et al. Immunohistochemical localization of avian leukosis virus subgroup J in tissues from naturally infected chickens. Avian Dis, 2001, 45(4): 992 - 998.
  • 6Payne L N. HPRS-103: a retrovirus strikes back. The emergence of subgroup J avian leukosis virus. Avian Pathology, 1998, 27(Supp1 ): S36 - S45.
  • 7Rainey G J A, Natonson A, Maxfield L F, et al. Mechanism of avian retrovirus host range extension. Journal of Virology, 2003, 77(12): 6709 - 6719.
  • 8Gingerich E, Porter R E, Lupiani B, et al. Diagnosis of myeloid leukosis induced by a recombinant avian leukosis virus in commercial white leghorn egg laying flocks. Avian Dis, 2002, 46 ( 3 ): 745 -748.
  • 9甘孟侯主编.禽病学.北京:中国农业出版社,2000,35-38.
  • 10Burmester, B.R. 1947. Studies on the transmission of avian visceral lymphomatosis. Ⅱ. Propagation of lymphomatosis with cellular and cell-free preparations. Cancer Res 7: 786-797.

共引文献177

同被引文献109

引证文献7

二级引证文献27

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部