摘要
目的观察菩人丹超微粉(Pu Ren Dan Superfine Powder,PRD)对2型糖尿病(Type 2 Diabetes Mellitus,T2DM)大血管病变大鼠骨骼肌组织胰岛素受体底物-1(insulinreceptor substrate-1,IRS-1)mRNA及葡萄糖转运蛋白4(glucose transporter4 GLUT4)mRNA表达的影响,探讨PRD调控骨骼肌葡萄糖转运的分子机制。方法以Wistar大鼠造T2DM模型大鼠,造模成功的T2DM大鼠按血糖结合体质量分层随机分为5组:模型组、罗格列酮组、PRD低、中、高剂量(0.885g/kg;1.770g/kg;3.540g/kg)治疗组,另取10只大鼠作为正常对照组。各组动物每日灌胃给药1次,正常对照组和T2DM模型组灌胃等剂量蒸馏水,连续给药4周。实验期间除正常对照组给予普通饲料外,其余各组均给予高脂高热量饲料。采用反转录PCR(Reverse Transcription-Polymerase Chain Reaction,RT-PCR)技术和凝胶电泳成像分析方法,观察T2DM大血管病变大鼠骨骼肌组织IRS-1、GLUT4mRNA表达。结果 T2DM大血管病变大鼠骨骼肌组织IRS-1、GLUT4mRNA表达与正常对照组比较明显降低(P<0.01);PRD治疗组均能提高T2DM大鼠骨骼肌组织IRS-1、GLUT4mRNA的表达(P<0.05,P<0.01)。结论 PRD可提高T2DM大血管病变大鼠骨骼肌IRS-1mRNA及GLUT-4mRNA的表达水平。
Objective To investigate the effects of ＂Pu Ren Dan Superfine Powder＂ on the expressions of insulin receptor substrate-1（IRS-1） and glucose transporter 4（GLUT4） in skeletal muscles of type 2 diabetic macroangiopathy rats and the molecular mechanism of the Powder in regulating glucose transport on skeletal muscles.Methods The RT-PCR technology and Gel electrophoresis image analysis methods were used to observe the expressions of IRS-1 and GLUT4 mRNA in skeletal muscles of type 2 diabetic macroangiopathy rats.Results The expressions of IRS-1 and the GLUT4 mRNA in skeletal muscles of type 2 diabetic macroangiopathy rats were significantly decreased compared with the control group（P〈0.01）.The Powder could increase the expressions of IRS-1 and GLUT4 mRNA in skeletal muscles of type 2 diabetic macroangiopathy rats（P〈0.05,P〈0.01）.Conclusion ＂Pu Ren Dan Superfine Powder＂ can increase the expressions of IRS-1 and（GLUT4） in skeletal muscles of type 2 diabetic macroangiopathy rats.
出处
《上海中医药杂志》
2010年第10期66-69,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30572303)
关键词
菩人丹超微粉
2型糖尿病
胰岛素受体底物-1
葡萄糖转运蛋白4
＂Pu Ren Dan Superfine powder＂
type 2 diabetes mellitus
insulin receptor substrate-1（IRS-1）
glucose transporter 4（GLUT4）
