缺氧复氧对血管内皮细胞ICAM-1表达及内皮-白细胞粘附的影响

Anoxia/reoxygenation increased the expression of ICAM1 and the adhesion between endothelial cells and neutrophils

目的：评价缺氧／复氧后血管内皮细胞ＩＣＡＭ１表达及内皮白细胞粘附率的变化。方法：体外培养大鼠骨髓血窦内皮细胞，缺氧９０ｍｉｎ，分别给予复氧１ｈ，２ｈ，４ｈ，８ｈ，１２ｈ，２４ｈ，用半定量ＥＬＩＳＡ法测ＩＣＡＭ１的表达量，同时还观察内皮细胞对中性粒细胞的粘附率。在复氧时加入药物ＮＡＣ、灯盏花，复氧后４ｈ观察ＩＣＡＭ１的表达。结果：（１）体外培养的内皮细胞缺氧后ＩＣＡＭ１表达上调，随复氧时间的延长ＩＣＡＭ１呈增强趋势。（２）药物灯盏花、ＮＡＣ对复氧４ｈ的ＩＣＡＭ１表达无抑制。（３）内皮细胞对白细胞的粘附率在缺氧后增加，复氧使粘附更强。ＩＣＡＭ１抗体或ＣＤ１１ｂ抗体抑制复氧后的粘附增强，而灯盏花、ＮＡＣ则无抑制作用。结论：（１）缺氧／复氧导致血管内皮细胞ＩＣＡＭ１表达上调，加强内皮白细胞粘附。（２）复氧时加入灯盏花、ＮＡＣ不能抑制ＩＣＡＭ１表达。

Objective: To evaluate the expression of ICAM1 and the adhesive rate of neutrophils with endothelial cells after anoxia and reoxygenation. Method: The endothelial cells of rat bone marrow were cultured in vitro.Endothelial cells monolayers were exposed to anoxia for 90 min,reoxygenate for 1h,2h, 4h,8h,12h,24h,expression of ICAM1 was assayed by ELISIA,and the adhesion between endothelial cells and neutrophils were measured.The endothelial cells monolayers also were treated with handmazz or NAC after reoxygenation. Results :(1)Increased expression of ICAM1 appeared in cultured endothelial cells after anoxia and enhanced after reoxygenation.(2)Handmazz or NAC couldn't inhibit ICAM1 expressin after reoxygenation 4h.(3)The adhesive rate of neutrophils with endothelial cells increased by anoxia and reoxygenation.AntiICAM1 antibody or CD11b antibody could suppress it,but handmazz or NAC had no affect. Conclusion:5BZ(1)The increased expression of ICAM1 and adhesion between endothelial cells and neutrophils were induced by anoxia/reoxygenation.(2)Treated with handmazz or NAC after oxygenation couldn't inhibit ICAM1 expression.