摘要
目的研究Ｒｂ、ｐ１６基因表达的蛋白产物在大肠癌组织中的变化及其意义。方法应用免疫组化ＳＰ法，在４０例原发性大肠癌中检测Ｒｂ、ｐ１６蛋白的表达。结果Ｒｂ蛋白表达阳性率为７２．５％（２９／４０）；ｐ１６蛋白表达阳性率为３７．５％（１５／４０）。２３例ｐ１６蛋白呈阴性或弱阳性表达的大肠癌组织中，Ｒｂ蛋白均显示阳性或强阳性，９例Ｒｂ蛋白呈阴性或弱阳性表达的大肠癌中，ｐ１６蛋白呈阳性或强阳性表达。１５例淋巴结转移的大肠癌中仅２例ｐ１６阳性，而２５例无转移者有１３例ｐ１６阳性。结论大肠癌发生过程中，存在Ｒｂ、ｐ１６蛋白表达的异常，Ｒｂ失活与ｐ１６表达之间存在负相关关系。ｐ１６蛋白表达缺失与大肠癌淋巴结转移有关。
Objective To study the expression of Rb,p16 and theirs significance in large intestinal carcinoma. Methods Immunohistochemistry was used to examine the proteins in 40 primary large intestinal carcinoma. Results The expression of Rb was revealed in 29/40 samples (72.5%) and of p16 was in 15/40 samples (37.5%). The expression of p16 in large intestinal carcinoma with lymph node metastasis was very low, only 2/15. In the 23 cases with p16 protein negative or weak positive, Rb showed positive or strong positive, while 9 cases of Rb negative or weak positive carcinomas showed high levels of p16. Conclusion The abnormal expression of Rb and p16 was accompanied by the happening of large intestinal carcinoma. The abscense of p16 expression was related to metastasis of lymph node in large intestinal carcinoma, while there was a reciprocity between Rb inactivation and p16 expression.
出处
《肿瘤研究与临床》
CAS
1999年第3期152-154,共3页
Cancer Research and Clinic
