黄芩苷对小鼠实验性结肠炎的保护作用

The protective effects of baicalin on experimental colitis in mice

目的:观察黄芩苷对三硝基苯磺酸(TNBS)致小鼠实验性结肠炎的保护作用,并探讨其作用机制。方法:将Balb/c小鼠分为4组:乙醇对照组、TNBS模型组、黄芩苷低剂量组和黄芩苷高剂量组。用TNBS灌肠诱导小鼠实验性结肠炎模型,TNBS灌肠2h后,灌胃给予黄芩苷,qd×7d。第8天处死动物,观察结肠黏膜的损伤程度,测定结肠髓过氧化物酶(MPO)活性以及丙二醛(MDA)和谷胱苷肽(GSH)的含量。结果:与TNBS模型组比较,黄芩苷低、高剂量组小鼠体质量、大体损伤评分及组织学表现均显著改善,MPO酶活性显著降低,MDA含量减少,谷胱苷肽含量增加。结论:黄芩苷可减轻TNBS诱导的小鼠实验性结肠炎,作用机制与黄芩苷的抗氧化作用有关。

OBJECTIVE To observe the effects of baicalin on experimental colitis induced by trinitrobenzenesulfonic acid （TNBS） in mice. METHODS Balb/c mice was randomly divided into four groups： ethanol control,TNBS model group and ba icalin treatment group （mice was treated with 40 and 8/1 mg·kg ^-1 baicalin）. Experimental colitis was induced by TNBS. baicalin was given by oral administration,qd 7,after TNBS treatment for 2 h. Mice was euthanized on eighth day. The histological and microscopical changes of colon were observed and activity of myeloperoxidase （MPO） and contents of malondialdehyde （MDA）,gluthathione （GSH） were evaluated. RESULTS The histological and microscopical changes of colon and changes of body weight in mice were significantly improved after treatment with 40 and 80 mg·kg ^-1 baicalin, compared with TNBS model group. Meanwhile, the activity of MPO and the content of MDA was reduced, respectively, the content of GSH was increased re markably in baicalin treatment group. CONCLUSION Baicalin can relieve the symptoms of experimental colitis induced by TNBS through the inhibition of oxidation.