HPLC法同时测定厚朴温中滴丸中5个有效成分的含量

HPLC simultaneous determination of five bioactive-components in Houpu Wenzhong drop-pills

目的:建立反相高效液相色谱法同时测定厚朴温中滴丸中木香内酯、去氢木香内酯、6-姜酚、和厚朴酚与厚朴酚的含量。方法:采用ZORBAX SB-C18色谱柱(250mm×4.6mm,5μm),以甲醇-水(60∶40)为流动相,流速1.0mL.min-1,检测波长250nm,柱温30℃。结果:5个成分均达到基线分离,木香内酯、去氢木香内酯、6-姜酚、和厚朴酚、厚朴酚的线性范围分别为0.0633～0.633μg(r=0.9998),0.0822～0.822μg(r=0.9997),0.0701～0.701μg(r=0.9998),0.0461～0.461μg(r=0.9997),0.0482～0.482μg(r=0.9998);平均回收率(n=6)分别为97.62%,97.81%,97.16%,97.32%,96.55%。结论:本方法简单、可靠、专属性强,可以作为控制厚朴温中滴丸质量的方法。

Objective：To establish HPLC method for the simultaneous determination of costunolide,dehydrocostunolide,6-gingerol,honokiol and magnolol in Houpu Wenzhong drop-pills.Methods：ZORBAX SB-C18（250 mm×4.6 mm,5 μm） column was adopted with methanol-water（60：40） as a mobile phase.The flow rate was 1.0 mL·min-1,and the detecting wavelength was 250 nm.The column temperature was at 30 ℃.Results：The five compounds were isolated.The linear ranges of costunolide,dehydrocostunolide,6-gingerol,honokiol and magnolol were 0.0633-0.633 μg（r=0.9998）,0.0822-0.822 μg（r=0.9997）,0.0701-0.701 μg（r=0.9998）,0.0461-0.461 μg（r=0.9997）,0.0482-0.482 μg（r=0.9998）;The average recoveries（n=6） were 97.62%,97.81%,97.16%,97.32%,96.55%.Conclusion：The method is reliable,accurate and specific.It can be used for quality control of Houpu Wenzhong drop-pills.