HPLC法测定小儿磨积片中橙皮苷、甘草酸、和厚朴酚、厚朴酚及苍术素的含量

RP-HPLC analysis of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin in Xiao'er Moji tablets

目的:建立HPLC法同时测定小儿磨积片中橙皮苷、甘草酸、和厚朴酚、厚朴酚及苍术素的含量。方法:采用DIKMADI-MONSILC18(4.6mm×250mm,5μm)色谱柱,以80%乙腈(A)-含0.2%磷酸及5%乙腈的水溶液(B)为流动相进行梯度洗脱(0～10min,80%B→70%B;10～20min,70%B→50%B;20～40min,50%B→5%B;40～50min,5%B→0%B),流速1.0mL.min-1,检测波长0～15min时283nm(橙皮苷)、16～30min时250nm(甘草酸)、31～40min时294nm(和厚朴酚、厚朴酚)、41～50min时335nm(苍术素),柱温40℃。结果:橙皮苷、甘草酸、和厚朴酚、厚朴酚及苍术素的线性范围分别为0.012～3.06,0.009～2.23,0.004～0.992,0.004～0.948,0.0005～0.123μg(r≥0.9990);平均回收率(n=6)分别为95.8%(RSD=1.9%),96.4%(RSD=1.2%),98.3%(RSD=1.7%),98.2%(RSD=1.6%),100.4%(RSD=1.3%)。结论:所建立的HPLC法操作简便,结果准确,重复性好,可同时测定小儿磨积片中橙皮苷、甘草酸、和厚朴酚、厚朴酚及苍术素的含量。

Objective：To develop an HPLC method for determination of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin in Xiao＇er Moji tablets.Methods：The analysis was carried out on a DIKMA DIMONSIL C18（4.6 mm×250 mm,5 μm） column;The mobile phases were 80% acetonitrile（A）-aqueous solution containing 0.2% phosphoric acid and 5% acetonitrile（B） with gradient elution（0-10 min,80% B→70% B;10-20 min,70% B→50% B;20-40 min,50% B→5% B;40-50 min,5% B→0% B） and the flow rate was 1.0 mL·min-1;The detection wavelengths were set at 283 nm for hesperidin（0-15 min）,250 nm for glycyrrhizic acid（16-30 min）,294 nm for honokiol and magnolol（31-40 min）,and 335 nm for atractylodin（41-50 min）;The column temperature was 40 ℃.Results：The linear ranges of hesperidin,glycyrrhizic acid,honokiol,magnolol and atractylodin were 0.012-3.06,0.009-2.23,0.004-0.992,0.004-0.948,0.0005-0.123 μg（r≥0.9990,respectively）;The average recoveries（n=6） were 95.8%（RSD=1.9%）,96.4%（RSD=1.2%）,98.3%（RSD=1.7%）,98.2%（RSD=1.6%）,100.4%（RSD=1.3%）,respectively.Conclusion：This method is simple and can be used to determine the above 5 components with satisfactory accuracy and repeatability.