土茯苓药材中落新妇苷和总黄酮的含量测定方法研究

Study on the determination of astilbin and total flavonoids in Rhizoma Smilacis Glabrae

目的:建立土茯苓药材中落新妇苷和总黄酮的含量测定方法。方法:采用HPLC法测定落新妇苷的含量,使用Shim-pack VP-ODS(150mm×4.6mm,5μm)色谱柱,流动相为乙腈-水-冰醋酸(18∶82∶1),流速1.0mL.min-1,检测波长290nm;采用比色法测定总黄酮的含量,以10%硝酸铝溶液为显色剂,测定波长为500nm。结果:在选定条件下,落新妇苷测定的线性范围为0.04～2.0μg(r=1.000),平均加样回收率为99.2%(n=9);总黄酮测定的线性范围为0.2～1.0mg(r=0.9998),平均加样回收率为101.8%(n=9)。结论:本文方法简便、准确,可以用于土茯苓药材中落新妇苷和总黄酮的含量测定。

Objective：To establish methods for determination of astilbin and total flavonoids in Rhizoma Smilacis Glabrae,respectively.Methods：An HPLC method was developed for quantitative determination of astilbin in Rhizoma Smilacis Glabrae.The analysis was performed on a Shim-pack VP-ODS column（150 mm×4.6 mm,5 μm） with the mobile phase consisting of acetonitrile-water-glacial acetic acid（18：82：1）.The flow rate was 1.0 mL·min-1 and the detection wavelength was 290 nm.A colorimetric method was used to determine the total flavanoids in Rhizoma Smilacis Glabrae.Total flavanoids were measured with 10% aluminum nitrate solution as color developing reagent and detection wavelength of 500 nm.Results：Astilbin had good linearity in the range of 0.04-2.0 μg（r=1.000）,the average recovery was 99.2%（n=9）.Total flavonoids had good linear relationship in the range of 0.2-1.0 mg（r=0.9998）,the average recovery was 101.8%（n=9）.Conclusion：The methods are simple,accurate and applicable for the quantitative determination of astilbin and total flavonoids in Rhizoma Smilacis Glabrae.