HPLC法测定肠炎宁糖浆中鸡屎藤苷甲酯的含量

HPLC determination of scandoside methyl ester in Changyanning syrup

目的:建立高效液相色谱法测定肠炎宁糖浆中鸡屎藤苷甲酯的含量。方法:采用Agilent Eclipse XDB-C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(13∶87),流速1.0mL.min-1,检测波长235nm。结果:鸡屎藤苷甲酯浓度在0.09～1.84mg·mL-1范围内,与峰面积呈良好线性关系;鸡屎藤苷甲酯平均回收率(n=6)为103.5%,RSD为1.9%。结论:本方法准确、简便,可用于测定肠炎宁糖浆中鸡屎藤苷甲酯的含量。

Objective：To develop an HPLC method for determination of scandoside methyl ester in Changyanning syrup. Methods：The separation was performed on an Agilent Eclipse XDB-C18（250 mm×4.6 mm,5 μm）column with a mobile phase of methanol-water（13：87）.The flow rate was 1.0 mL·min-1,and the detecting wavelength was 235 nm. Results：The linear range of scandoside methyl ester was 0.09-1.84 mg·mL-1,and the average recovery（n=6）was 103.5% with RSD of 1.9%.Conclusion：The proposed method is simple,rapid and accurate for the determination of scandoside methyl ester in Changyanning syrup.