摘要
目的:建立高效液相色谱法测定肠炎宁糖浆中鸡屎藤苷甲酯的含量。方法:采用Agilent Eclipse XDB-C18(250mm×4.6mm,5μm)色谱柱,流动相为甲醇-水(13∶87),流速1.0mL.min-1,检测波长235nm。结果:鸡屎藤苷甲酯浓度在0.09~1.84mg·mL-1范围内,与峰面积呈良好线性关系;鸡屎藤苷甲酯平均回收率(n=6)为103.5%,RSD为1.9%。结论:本方法准确、简便,可用于测定肠炎宁糖浆中鸡屎藤苷甲酯的含量。
Objective:To develop an HPLC method for determination of scandoside methyl ester in Changyanning syrup. Methods:The separation was performed on an Agilent Eclipse XDB-C18(250 mm×4.6 mm,5 μm)column with a mobile phase of methanol-water(13:87).The flow rate was 1.0 mL·min-1,and the detecting wavelength was 235 nm. Results:The linear range of scandoside methyl ester was 0.09-1.84 mg·mL-1,and the average recovery(n=6)was 103.5% with RSD of 1.9%.Conclusion:The proposed method is simple,rapid and accurate for the determination of scandoside methyl ester in Changyanning syrup.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2010年第9期1760-1762,共3页
Chinese Journal of Pharmaceutical Analysis