仿生提取-HPLC-ICP-MS测定含雄黄中成药中可溶性砷含量

Bionic extraction-HPLC-ICP-MS determination of soluble arsenic in Chinese traditional patent medicine containing realgar

目的:建立仿生提取-高效液相色谱-电感耦合等离子体质谱联用技术(HPLC-ICP-MS)测定含雄黄中药制剂中可溶性砷形态含量的分析测定方法。方法:通过模拟人体胃肠道环境,采用人工胃液提取含雄黄中药制剂中的可溶性砷,结合HPLC-ICP-MS进行分离测定。结果:应用阴离子色谱柱10min内成功分离4种形态砷——亚砷酸盐As(Ⅲ)、砷酸盐As(Ⅴ)一甲基砷(MMA)和二甲基砷(DMA)。方法的重复性良好,RSD为1.4%～1.5%;4种砷形态:亚砷酸盐As(Ⅲ)、砷酸盐As(Ⅴ)一甲基砷(MMA)和二甲基砷(DMA)的检出限分别为:0.10,0.10,0.10,0.20μg.L-1。结论:方法简便、灵敏、准确、重现性好,可用于含雄黄中药制剂中可溶性砷形态的分析测定。

Objective：To establish a method for determination of soluble arsenic in Chinese traditional patent medicine containing realgar by bionic extraction-HPLC-ICP-MS.Method：Through simulating stomach conditions,the arsenic species were extracted by artificial gastric juice and separated by high-pressure liquid chromatography （HPLC） with ICP-MS detection.Results：Separation of four arsenic species-As（Ⅲ）,As（Ⅴ）,MMA,DMA-was achieved with a anion-exchange column.The entire separation was accomplished in 10 min.The precision was calculated to be 1.4%-1.5% （RSD） for arsenic species.The HPLC-ICP-MS detection limits for the four arsenic species were in 0.10-0.20 μg·L-1.Conclusion：The developed method is rapid,sensitive and accurate.It can be used for determination of arsenic species in Chinese traditional patent medicine.