HPLC法测定仙蟾胶囊中华蟾酥毒基及脂蟾毒配基的含量

HPLC determination of cinobufagin and resibufogenin in Xianchan capsules

目的:建立HPLC法测定仙蟾胶囊中华蟾酥毒基及脂蟾毒配基含量。方法:采用高效液相色谱法,色谱柱为Agilent ZORBAXSB-C18(250mm×4.6mm,5μm),流动相为乙腈(A)-水(B),梯度洗脱,流速1.0mL.min-1,检测波长296nm,柱温40℃。结果:华蟾酥毒基及脂蟾毒配基分别在4.0～100.0μg.mL-1(r=0.9999)及2.0～100.2μg.mL-1(r=0.9999)范围内线性关系良好,平均回收率(n=6)分别为101.6%(RSD=1.5%)和97.65%(RSD=1.6%)。结论:本法操作简便、准确,重复性好,可用于仙蟾胶囊的质量控制。

Objective：To develop an HPLC method for the determination of cinobufagin and resibufogenin in Xianchan capsules.Methods：The chromatographic separation was achieved on an Agilent ZORBAX SB-C18（250 mm×4.6 mm,5 μm） column;The mobile phase was acetonitrile（A）-water（B） with gradient elution at the flow rate of 1.0 mL·min-1.The wavelength was 296 nm and the column temperature was 40 ℃.Results：The calibration curves were linear in the ranges of 4.0-100.0 μg·mL-1（r=0.9999） for cinobufagin and 2.0-100.2 μg·mL-1（r=0.9999） for resibufogenin.The recoveries（n=6） of cinobufagin and resibufogenin were 101.6%（RSD=1.5%） and 97.65%（RSD=1.6%）.Conclusion：The method is simple and accurate with good reproducibility,and can be used for the quality control of Xianchan capsules.