尪痹片定性定量方法的研究

Study on qualitative and quantitative methods for Wangbi tablets

目的:建立尪痹片中独活、白芍、知母的鉴别方法及淫羊藿的淫羊藿苷含量测定方法.方法:采用薄层色谱法对尪痹片中独活、白芍、知母进行定性鉴别;采用高效液相色谱法对尪痹片中淫羊藿的淫羊藿苷进行含量测定,以Agilent C18(4.6mm×250mm,5μm)柱为分析柱,甲醇-水-冰醋酸(55:44:1)为流动相,流速1.0mL.min-1,检测波长270nm。结果:薄层色谱中均能明显地检出独活、白芍中的芍药苷、知母中的菝契皂苷元;淫羊藿苷浓度在0.027～0.68μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=9)为100.0%。结论:本法简便、准确,专属性强,可以作为尪痹片的定性定量方法。

Objective：To establish a method for identification of Angelicae Biserratae Radix,Paeoniae Alba Radix and Anemarrhenae Rhizome,and a method for content determination of icariin in Wangbi tablets.Methods：Angelicae Biserratae Radix,Paeoniae Alba Radix and Anemarrhenae Rhizome were identified by TLC;The HPLC for determination icariin of Wangbi tablets was conducted on an Agilent C18（4.6 mm×250 mm,5 μm） with methanol-water-glacial acetic acid（55：44：1） as mobile phase at the flow rate of 1.0 mL·min-1,and detection wavelength was set at 270 nm.Results：Angelicae Biserratae Radix,peoniflorin of Paeoniae Alba Radix and sarsasapogenin of Anemarrhenae Rhizome were identified by TLC apparently.The calibration curve of was linear range of 0.027-0.68 μg（r=0.9999）,and the average recovery（n=9） of icariin was 100.0%.Conclution：The method is simple,accurate,and suitable for the determination of Wangbi tablets.