HPLC法测定大叶紫珠中毛蕊花糖苷的含量

HPLC determination of verbascoside in Callicarpae Macrophyllae Folium

目的：建立反相高效液相色谱法测定大叶紫珠中毛蕊花糖苷的含量。方法：采用Agilent Eclipse XDB-C18色谱柱（250mm×4.6mm,5μm）,以乙腈-0.5%磷酸（18：82）为流动相,流速1.0mL.min-1,检测波长332nm,柱温30℃。结果：毛蕊花糖苷进样浓度在12.01～240.2μg.mL-1范围内,与峰面积呈良好线性关系;平均回收率（n=6）为99.6%,RSD为1.4%。结论：该方法简便、准确,重复性好,可作为大叶紫珠的质量控制方法。

Objective：To develop an RP-HPLC method for the determination of verbascoside in Callicarpae Macrophyllae Folium.Methods：The separation was performed at 30 ℃ on an Agilent Eclipse XDB-C18 column（250 mm×4.6 mm,5 μm） with a mobile phase of acetonitrile-0.5% phosphoric acid（18：82）.The flow rate was 1.0 mL·min-1,and the detecting wavelength was 332 nm.Results：The linear range of verbascoside was from 12.01 to 240.2 g·mL-1;The average recovery（n=6） of verbascoside was 99.6%（RSD=1.4%）.Conclusion：The method is simple and accurate with good reproducibility.It can be used to control the quality of Callicarpae Macrophyllae Folium.