高效液相-库仑阵列电化学法测定对乙酰氨基酚唾药浓度

HPLC-MS determination of paracetamol concentration in human saliva

目的:建立用高效液相-库仑阵列电化学检测器测定唾液中对乙酰氨基酚浓度的方法。方法:受试者服用对乙酰氨基酚片,分别于不同时间点取受试者唾液经处理,以甲醇(加5%的水相)与水相(0.03mol.L-1磷酸二氢钠溶液,氢氧化钠溶液和磷酸溶液调pH至6.0)(20:80)为流动相,流速1mL.min-1,350mV电压下检测。结果:对乙酰氨基酚在8.3～833.3ng.mL-1范围内线性关系良好,最低检测限为3.3ng.mL-1,平均回收率、重复性等均符合方法学要求。结论:高效液相-库仑阵列电化学法操作简便、准确,灵敏度高,适用于对乙酰氨基酚体内代谢研究。

Objective：To develop an HPLC-ECD mothod for determination of paracetamol concentrations in human saliva.Method：The objects take paracetamol tablet,saliva were collected by regularly time,then concentration of paracetamol was determined by HPLC-electrochemical detector and mobile phase of methanol（5% 0.03 mol·L-1 NaH2PO4） and water（0.03 mol·L-1 NaH2PO4,pH=6.0） with a ratio of 20：80 at flow rate of 1 mL· min-1,the detection voltage was 350 mV.Results：The content of paracetamol showed good linearity in the range of 8.3-833.3 ng·mL-1.The lowest limit of quantity was 3.3 ng·mL-1,the average recovery and reproducibility of related date appeared to be good.Conclusion：Electrochemical detection is convenient,accurate and sensitivity,It can be used for the study of paracetamol in vivo metabolism.