人参药材中有机氯农药残留量测定前处理方法比较

The Comparison of Organochlorine Pesticide Residues in Ginseng Determined by Many Different Fore Treatment Methods

目的采用4种前处理方法对人参药材进行有机氯农药残留量测定,以此对比不同方法间的提取差异。方法分别采用《中国药典》2005年版方法、混合小柱净化方法、凝胶制备色谱(GPC)净化方法、SPE(Florisil)小柱净化方法测定,并分别进行了回收率计算。结果采用《中国药典》2005年版方法、混合小柱净化方法测定回收率在60%～140%之间。结论分析了4种前处理方法对人参药材中有机氯农药残留量测定的影响,为优化实验条件提供依据。

Objective Organochlorine pesticide residues in Ginseng were determined by four different fore treatment methods,so that contrast the differences of the methods. Methods ＂Chinese Pharmacopoeia＂ 2005 edition method,mixing a small column purification,gel chromatography （GPC） purification and SPE （Florisil） were determined,and recovery rates were calculated respectively. Results Recovery rates of ＂Chinese Pharmacopoeia＂ 2005 edition method and mixing a small column purification were determined between 60% to 140%. Conclusion The theoretical basis on analyzing the contribution of organochlorine pesticide residues in Ginseng by four fore treatment methods was provided to optimize the experiment condition.