自然流产夫妇巨细胞病毒抗体测定的临床意义

Detection of antibodies for human cytomegalovirus in the couples with spontaneous abortion and their clinical significance

目的：探讨自然流产病人ＨＣＭＶ抗体测定的临床意义。方法：用市售ＨＣＭＶ－Ｉｇ的间接ＥＬＩＳＡ试剂盒测定病人血清中的ＨＣＭＶ－Ｉｇ滴度。结果：４２例ＲＳＡ病人、３３例早孕对照组、３７例正常对照组ＨＣＭＶ－ＩｇＧ、ＨＣＭＶ－ＩｇＭ抗体测定结果为：１０倍稀释的血清ＨＣＭＶ－ＩｇＧ在３组标本中的检出率分别为８８．１％、８７．９％和８３．５％；血清ＨＣＭＶ－ＩｇＭ抗体检出率分别为８３．３％、６６．７％和６７．７％；血清１００倍稀释时，ＲＳＡ组两种类别ＨＣＭＶ抗体的阳性检出率分别为２８．６％和３８．１％，对照组的ＨＣＭＶ－ＩｇＧ检出率分别为１５．２％和２．７％；ＨＣＭＶ－ＩｇＭ分别为６．１％和２．７％；血清１０００倍稀释，ＨＣＭＶ的两种类别抗体仅实验组有较低的检出率（分别为９．５％和７．１％）外，两个对照组均未检出阳性病例。结论：早孕妇女反复自然流产与ＨＣＭＶ感染有密切关系，对早孕妇女进行ＨＣＭＶ抗体调查时，血清１∶１０００稀释对结果的判定有重要价值。

Objectives: To explore the clinical significance of antibodies for human cytomegalovirus(HCMV) in spontaneous abortion patients. Methods: Titer of HCMVIg in patients sera were detected using commerical indirect ELISA Kit. Results: Fortytwo RSA patients,33 early pregnant women(as control group) and 37 normal controls were studied.The positive rates of HCMVIgG in patients sera diluted by 10 times were 88.1%,87.9% and 83.5% respectively in the three groups,while HCMVIgM were 83.3%,66.7% and 67.7% respectively.When sera were diluted by 100 times,the positive rates of the two kind of antibodies for HCMV were 28.6% and 38.1% in RSA group,while HCMVIgG were 15.2% and 2.7% in the two control groups respectively.When sera were diluted by 1 000 times,the lower positive rates(9.5% and 7.1%) of two kind of antibodies for HCMV were only found in RSA group,but no positive cases were found in two control groups. Conclusions: RSA is closely associated with infection of HCMV in early pregnant women.Chosing suitable CytomegalovirusAntibodydilution of sera(11 000) is important to judge the results of detection for HCMV antibodies in early pregnant woman.