披针新月蕨细胞毒活性成分研究

Constituents from Abacopteris penangiana and their cytotoxic activity

目的研究民族药披针新月蕨Abacopteris penangiana化学成分并评价其细胞毒活性。方法以硅胶柱、凝胶柱色谱分离,制备HPLC纯化,采用MS,1、2 D-NMR等波谱方法进行结构鉴定,MTT法测试各化合物的细胞毒活性。结果从披针新月蕨中分离鉴定了6个化合物,分别为4(S),5,7-三羟基-4′-甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷[4(S),5,7-trihydroxy-4′-methoxy-6,8-di methyl-2(R)-flavan-5,7-O-β-D-diglucopyrano-side,eruberin B,1];5,7-二羟基-4(S),4′-二甲氧基-6,8-二甲基-2(R)-黄烷-5,7-O-β-D-二葡萄糖苷[5,7-di-hydroxy-4(S),4′-di methoxy-6,8-di methyl-2(R)-flavan-5,7-O-β-D-diglucopyranoside,eruberin C,2];5,7-二羟基-4′-甲氧基-6-羟甲基-8-甲基-2″,4(S)-氧-2(R)-黄烷-5-O-β-D-葡萄糖苷[5,7-dihydroxy-4′-methoxy-6-hydroxymethyl-8-methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,3];5,7-二羟基-4′-甲氧基-6,8-二甲基-2″,4(S)-氧-2(R)-黄烷-5-β-D-葡萄糖苷[5,7-dihydroxy-4′-methoxy-6,8-di methyl-2″,4(S)-oxido-2(R)-flavan-5-O-β-D-glucopyranoside,eruberin A,4];高圣草素[5,7,4′-trihydroxy-3′-methoxyflavanone,homoeriodictyol,5];对-甲氧基桂皮醛(p-methoxycinnamaldehyde,6)。化合物3、4对L929及HeLa细胞的IC50值分别约为13.05、24.75μg/mL,25.11、18.32μg/mL。结论化合物3为新的天然产物,化合物2、5、6首次从新月蕨属植物中分得,化合物3、4对L929和HeLa具有显著抑制活性。

Objective To investigate the constituents from Abacopteris penangiana and their cytotoxic activity.Methods The constituents were isolated with silica gel,gel permeation chromatography,and purified by HPLC.Their structures were elucidated by spectroscopy.The antitumor effects of the isolated compounds were studied by MTT method in vitro.Results Six compounds were isolated and elucidated as followings： [4（S）,5,7-trihydroxy-4′methoxy-6,8-dimethyl-2（R）-flavan-5,7-O-β-D-diglucopyranoside,eruberin B]（1）,[5,7-dihydroxy-4（S）,4′-dimethoxy-6,8-dimethyl-2（R）-flavan-5,7-O-β-D-diglucopyranoside,eruberin C]（2）,[5,7-dihydroxy-4′-methoxy-6-hydroxymethyl-8-methyl-2″,4（S）-oxido-2（R）-flavan-5-β-D-glucopyranoside]（3）,[5,7-dihydroxy-4′-methoxy-6,8-dimethyl-2″,4（S）-oxido-2（R）-flavan-5-β-D-glucopyranoside,eruberin A]（4）,[5,7,4′-trihydroxy-3′-methoxyflavanone,homoeriodictyol]（5） and p-methoxycinnamaldehyde（6）.Compounds 3 and 4 showed significant inhibition against L929（IC50 13.05 and 24.75 μg/mL） and HeLa （IC50 25.11 and 18.32 μg/mL） cell lines,respectively.Conclusion Compound 3 is a new natural product.Compounds 2,5 and 6 are isolated from this genus for the first time.Compounds 3 and 4 show significant inhibitory effect against L929 and HeLa.