摘要
目的建立鬼臼毒素醇质体包封率的测定方法。方法采用葡聚糖凝胶微柱离心法分离醇质体与游离药物;用HPLC法测定醇质体中鬼臼毒素,计算包封率。结果在本法选择的色谱条件下,鬼臼毒素得到良好的分离,辅料不干扰测定,鬼臼毒素在2.5~101.1μg/mL（r=0.999 9）线性关系良好。微柱离心法能有效的将鬼臼毒素醇质体与游离药物分离,用该方法测得鬼臼毒素醇质体的包封率为51.03%,RSD为1.24%（n=3）。结论建立了微柱离心-HPLC法,该方法便捷,准确,重现性好,可用于测定鬼臼毒素醇质体的包封率。
Objective To establish a method for determining the entrapment efficiency of podophyllotoxin in ethosomes.Methods Glucose gel minicolumn centrifugation was employed to separate the free drug from the ethosomes.The content of podophyllotoxin was quantified by HPLC.Results In the current chromatograph condition,the excipient had no influence on the determination of podophyllotoxin.The assay was linear during the podophyllotoxin concentration range of 2.5-101.1 μg/mL（r=0.999 9）.The ethosomes were well separated using the method of minicolumn centrifugation.The entrapment efficiency of podophyllotoxin-encapsulated ethosomes was 51.03%,with RSD 1.24%（n=3）.Conclusion The established minicolumn centrifugation-HPLC method is simple,accurate,reproducible,and applicable for the determination of entrapment efficiency of podophyllotoxin-encapsulated ethosomes.
出处
《中草药》
CAS
CSCD
北大核心
2010年第10期1634-1637,共4页
Chinese Traditional and Herbal Drugs
基金
教育部新世纪优秀人才支持计划项目(NECT08-0898)
上海市教委重点学科建设项目(J50302)
关键词
鬼臼毒素
醇质体
微柱离心
包封率
podophyllotoxin
ethosomes
minicolumn centrifugation
entrapment efficiency
