芹菜素对局灶性脑缺血再灌注大鼠脑内caveolin-1表达的影响

Effects of apigenin on caveolin-1 expression of focal cerebral ischemia-reperfusion in rats

目的探讨芹菜素对局灶性脑缺血再灌注大鼠脑内caveolin-1表达的影响。方法采用大脑中动脉栓塞法复制脑缺血再灌注模型,将动物随机分为假手术组、模型组、地塞米松组、芹菜素组,分别给予不同处理,在6 h、1d、3 d7、d不同灌注时间点处死动物,用5分法对大鼠神经行为学进行评定,脑组织TTC染色以及采用免疫组织化学法观察caveolin-1的表达。结果芹菜素能显著减轻神经功能缺损;神经行为学评分显示,芹菜素3 d组大鼠神经行为学评分明显低于模型组(P<0.05);TTC染色示模型组出现白色梗死灶;正常脑内有一定量的caveolin-1表达,缺血后caveolin-1迅速增加,分别于术后1 d或3 d达高峰,芹菜素组显著提高caveolin-1的表达,在各时间点芹菜素组与模型组差异显著(P<0.01)。结论芹菜素可调节脑缺血再灌注后caveolin-1的表达,对大鼠局灶性脑缺血再灌注损伤具有保护作用,是促进其神经功能恢复的可能机制之一。

Objective To investigate the effects of apigenin（APG） on expression of caveolin-1 of focal cerebral ischemia-reperfusion in rats.Methods Cerebral ischemia was induced by middle cerebral artery occlusion.The rats were randomly divided into four groups including Sham-operated,model,Dexamethasone,and APG groups.All the rats were killed in 6 h and 1,3 or 7 d after operation.Neurological beha-vior was assessed by five score methods.Brain slices were observed by TTC stainning,and the expression of caveolin-1 was measured by immunohistochemistry.Results APG could obviously reduce the deficit of nervous functions;Neurological behavior scores of the rats in 3 d APG group was significantly lower than those in ischemia model group（P0.05）.Typical cortical infarct lesions in model group were found by TTC stainning.The expression of caveolin-1 positive cells existed in normal brain,and caveolin-1 was enhanced immediately after ischemia and reached to peak at 1 or 3 d after operation,respectively;APG could increase the expressions of caveolin-1 positive cells at every time point after ischemia and treatment（P0.01）.Conclusion APG is capable of regulating the expression of caveolin-1 in ischemic brain and has the effects on prevention of focal cerebral ischemia-reperfusion injury in rats which may be one of anti-ischemic mechanisms of APG.