摘要
目的利用绞股蓝RAPD标记有效鉴别绞股蓝及其伪品。方法用19条引物对17个绞股蓝样品和一个乌蔹莓样品进行RAPD-PCR扩增,并对绞股蓝DNA特异条带克隆测序及序列分析。结果获得一个绞股蓝RAPD标记。经克隆测序,8个序列高度保守,片段长度在766~770 bp,相似性均在97%以上。结论获得的RAPD标记可以作为合成探针的基础,用于鉴别绞股蓝及其伪品。
Objective The study is to identify the medicinal plant Gynostemma pentaphyllum and its fake called Cayratia japonica through the RAPD markers.Methods Nineteen random primers screened were applied to amplifying the total DNA extracts of 18 samples with RAPD and the spectific fragments of G.pentaphyllum were analyzed by cloning and sequencing.Results A RAPD marker of G.pentaphyllum was acquired.The result of sequence analysis indicated that the length of eight sequences is 766-770 bp and the identities of them are over 97%.Conclusion The RAPD marker acquired could provide a basis for making probe to distinguish G.pentaphyllum and its spurious breed.
出处
《中草药》
CAS
CSCD
北大核心
2010年第10期1692-1696,共5页
Chinese Traditional and Herbal Drugs
基金
广西科学基金资助项目(桂科基0731065)
广西区教育厅硕士研究生科研创新项目(2008105981001M228)
关键词
绞股蓝
RAPD标记
序列分析
Gynostemma pentaphyllum（Thunb.） Markino
RAPD marker
sequence analysis
