摘要
目的：制备与分析抗可提取性核抗原（ＥＮＡ）单克隆抗体；方法：以ＥＮＡ为抗原，免疫ＢＡＬＢ／ｃ小鼠，取其脾细胞与小鼠Ｓｐ２／０骨髓瘤细胞融合，经反复筛选和再克隆化，建立能稳定分泌抗ＥＮＡ单克隆抗体（ＭｃＡｂｓ）的杂交瘤细胞株；结果：共获得１１株能稳定分泌抗ＥＮＡ的杂交瘤细胞株。对其中的５种单克隆抗体（ＩＧ５，２Ｅ５，２Ｃ６，４Ｃ１０和４Ｅ６）进行了初步分析，结果如下：小鼠腹水抗体效价范围２×１０－５～３×１０－６；免疫球蛋白亚类鉴定，１株为ＩｇＧ２ａ，１株为ＩｇＧ２ｂ，其余３株均为ＩｇＧ１；
Objective: ,5BZPreparationandcharacterizationofmonoclonalantibodiesagainstextractablenuclearantigen(ENA)5,5HZ.Methods: BALA/cmiceareimmunizedwithanENApreparation andtheimmunizedspenocytesarefusedwithmurineSp2/0myelomacells.Afterseveralsubcloningandscreening,hybridomassecretingmonoclonalantibodies(McAbs)againstENAstablyareestablished. Results: 11hybridomassecretingMcAbsagainstENAstablywereestablished.Preliminaryanalysesofthe5McAbs(IG5,2E5,2C6,4C10 and 4E6)wereconducted.Theresultswereobtainedasfollows:Ascitestiters,from2105to3106;Igsubclasses,oneIgG2a,oneIgG2bandthreeIgG1. Conclusions:ZAs aweakimmunogen,ENAcanbeusedinpreparingthemonoclonalantibody.
出处
《河北医学》
CAS
1999年第5期1-2,共2页
Hebei Medicine
关键词
杂交瘤
单克隆抗体
可提取性核抗原
Hybridoma
Monoclonalantibody
Extractablenuclearantigen(ENA)
