远志蔗糖酯类新化学成分的分离与鉴定

Separation and identification of new sucrose esters from root of Polygala tenuifolia Willd.

目的对远志根的化学成分进行研究。方法采用反复硅胶柱色谱、Sephadex LH-20柱色谱和半微量制备高效液相色谱分离纯化，根据ESI—MS、^1H-NMR和^13C—NMR等谱学数据进行结构鉴定。结果从远志根的乙醇提取物中分离得到11个单体化合物，分别鉴定为蔗糖酯类化合物西伯利亚远志糖A5（sibiricose A5，1）、tenuifolioside A（α-D—glucopyranoside，3-O-[（2Z）-3-（4-hydroxy-3-methoxyphenyl）-1-oxo-2-propen-1—yl]-β—D—fructofuranosyl，2）、tenuifoliosideB（a—D—glucopyranoside，3-D-[（2E）-3-（3，4-dimethoxyphenyl）-1-oxo-2-propen-1-yl]-β-D-fmctofuranosyl，3）、tenuifoliosidcC（d—D—glucopyranoside，3-O-[（2z）-3-（3，4-dimethoxyphenyl）-1-oxo-2-pmpen-1-yl]β-D-fmctofurano-syl，4）、西伯利亚远志糖氏（sibiricose A6，5）、球腺糖A（glomemtose A，6）；咕吨酮类化合物西伯利亚远志咕吨酮B（sibiricaxanthone B，7）、远志咕吨酮Ⅺ（polygalaxanthone Ⅺ，8）、1，2，3，7-四甲氧基咕吨酮（1，2，3，7-tetramethoxyxanthone，9）和远志酮Ⅱ（onjixanthone Ⅱ，10）；远志醇（polygitol，11）。结论化合物2～4为首次发现的新化合物。

Objective To study the constituents of the extract from the root of Polygala tenuifolia Willd. Methods The chemical constituents were isolated by silica, Sephadex LH-20 gel column chromatography and HPLC. Chemical characters and spectroscopic analysis were employed for the structural identification. Results Eleven compounds were obtained and their structures were identified as sibiricose A5 （ 1 ）, tenuifolioside A （ a-D-glucopyranoside, 3-O-[ （ 2Z ） -3-（ 4-hydroxy-3-methoxyphenyl ） -1-oxo-2-propen-l-yl ] -β-D-fructo- furanosy1,2 ）, tenuifolioside B （ α-D-glucopyranoside, 3-0-[ （2E） -3-（ 3,4-dimethoxyphenyl ） -1 -oxo-2-pro- pen-l-yl] -β-D-fructofuranosy1,3 ）, tenuifolioside C （ α-D-glucopyranoside, 3-O-[ （2Z） -3-（ 3,4-dimethoxyphenyl ） - 1 -oxo-2-propen- 1 -yl ] -fl-D-fructofuranosyl, 4 ）, sibiricose m6 （ 5 ） , glomeratose A （6）, sibiricaxantho- ne B （ 7 ）, polygalaxanthone Ⅺ （ 8 ）, 1,2,3,7-tetramethoxyxanthone （ 9 ）, onjixanthone 11 （ 10 ）, and polygitol （11）. Conclusions Compounds 2- 4, named tenuifolioside A- C respectively, are new sucrose esters which have not been reported before.