中药黄蜀葵花化学成分的分离与鉴定(Ⅱ)

Isolation and identification of chemical constituents from the flowers of Abelmoschus manihot(L.) Medic. (Ⅱ)

目的研究中药黄蜀葵(Abelmoschus manihot(L.)Medic.)花的化学成分,为进一步开发利用该植物资源提供依据。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析鉴定化合物的结构。结果从黄蜀葵花体积分数为95%的乙醇提取物的大孔吸附树脂洗脱物中分离鉴定了9个以槲皮素为母核的黄酮类化合物,分别为槲皮素(quercetin,1)、金丝桃苷(hyperoside,2)、槲皮素-3-O-β-D-葡萄糖苷(quercetin-3-O-β-D-glucopyranoside,3)、槲皮素-3-O-β-D-6″-乙酰葡萄糖苷(quercetin-3-O-β-D-6″-acetylglucopyr-anoside,4)、槲皮素-3-O-刺槐糖苷(quercetin-3-O-robinoside,5)、槲皮素-3-O-芸香糖苷(quercetin-3-O-rutinoside,6)、槲皮素-3-O-β-D-木糖基-(1→2)-β-D-半乳糖苷(quercetin-3-O-β-D-xylopyranosyl(1→2)-β-D-galactopyranoside,7)、槲皮素-3′-O-β-D-葡萄糖苷(quercetin-3′-O-β-D-glucopyranoside,8)以及槲皮素-7-O-β-D-葡萄糖苷(quercetin-7-O-β-D-glucopyranoside,9)。结论化合物4、5、7和9为首次从秋葵属植物中分离得到。

Objective To study the chemical constituents from the flowers of Abelmoschus manihot （L.） Medic. Methods The compounds were isolated by chromatography by using silica gel, ODS, Sephadex LH- 20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Nine flavonoids which have quercetin skeleton were isolated. The structures of them were identified as quercetin （ 1 ）, hyperoside （ 2 ）, quercetin-3-O-β-D-glucopyranoside （ 3 ）, quercetin-3-O-β-D-6-acetylglucopyranoside （4）, quercetin-3-O-robinoside （5）, quercetin-3-O-rutinoside （ 6 ）, quercetin-3-O-β-D-xylopyranosyl- （ 1 →2 ） - β-D-galactopyranoside （ 7 ）, quercetin-3 ＇-O-/3-D-glucopyranoside （ 8 ） and quercetin-7-O-β-D-glucopyranoside （9） ,respectively. Conclusions Compounds 4,5,7 and 9 are obtained from Abelmoschus genus for the first time.