杨梅叶总黄酮对培养大鼠皮质神经元缺糖缺氧损伤的影响

Effects of total flavonoids from the leaves of Myrica rubra on cultured rat cortical neurons during combined oxygen and glucose deprivation

目的探讨杨梅叶总黄酮对原代培养大鼠皮质神经元缺糖缺氧损伤的影响。方法采用原代培养的大鼠皮质神经元,随机分为正常组、神经元缺糖缺氧(oxygen-glucose deprivation,OGD)模型组和杨梅叶总黄酮高、中、低质量浓度(100、10、1 mg.L-1)给药组,MTT法观察神经元的存活率,比色法检测细胞培养液中乳酸脱氢酶(lactate dehydrogenase,LDH)释放量、过氧化氢(hydrogen perox-ide,H2O2)含量,并检测细胞内超氧化物歧化酶(superoxide dismutase,SOD)活性及丙二醛(malondi-aldehyde,MDA)含量。结果杨梅叶总黄酮可显著提高受损伤神经元的存活率和皮质神经元细胞内SOD活性,降低神经元细胞LDH的释放、细胞内MDA含量和细胞培养液中H2O2含量。结论杨梅叶总黄酮对缺糖缺氧所致皮质神经元损伤具有明显的保护作用。

Objective To investigate the effects of total flavonoids from the leaves of Myrica rubra on the primary cultured rat cortical neurons during combined oxygen and glucose deprivation. Methods Primary cultured rat cortical neurons were randomly divided into the normal control group, the model group and total flavonoids from the leaves of Myrica rubra on low-dose, medium-dose and high-dose （100 mg. L^-1, 10 mg.L^-1, 1 mg.L^-1）group. The cortical neurons viability was determined by MTT assay. LDH leakage, concentration of hydrogen peroxide （ H2O2 ）, the intracellular levels of superoxide dismutase （SOD） and malondialdelyde （MDA）production were assessed by chromatometry. Results Total flavonoids treatment significantly reduced LDH leakage, H2O2, MDA production and increased the cell viability and SOD levels in cortical neurons subjected to oxygen-glucose deprivation. Conclusions The results indicate that total flavonoids from the leaves of Myrica rubra strongly protect primary cultured neurons against oxygen-glucose deprivation-induced oxidative stress.