人参药材皂苷类成分UPLC特征图谱的质量评价方法

UPLC fingerprint for quality assessment of ginsenosides of Ginseng Radix et Rhizoma

建立人参药材皂苷类成分超高效液相色谱(UPLC)特征图谱的质量评价方法。以人参药材为研究对象,采用RP-UPLC法,色谱柱为ACQUITYUPLCBEHC18(50mm×2.1mmID,1.7μm),乙腈-水二元梯度洗脱模式,流速为0.3mL·min-1,检测波长为203nm,建立了11批不同产地人参药材皂苷类成分UPLC特征图谱。特征图谱有15个共有色谱峰,并采用对照品指认了其中的9个色谱峰。对不同产地的人参皂苷类成分特征图谱进行聚类分析和主成分分析,并用"中药色谱指纹图谱相似度评价系统(2004B版)"对其质量进行了评价。所建立的人参皂苷类成分的特征图谱特征性和专属性强,且该方法快速、简便、可靠,可用于全面控制人参的质量。

This paper is aimed to establish the method of fingerprint analysis of chemical constituents by reversed-phase ultra-performance liquid chromatography （UPLC） for the quality control of the roots and rhizomes of Panax ginseng （Ginseng Radix et Rhizoma）. The method was performed on a ACQUITY UPLC BEH ClS （50 mm × 2.1 mm ID, 1.7 μm） with a mixed mobile phase of water and acetonitrile in a gradient mode. The flow rate was 0.3 mL·min^-1 and the wavelength of measurement was 203 nm. Eleven batches of the Ginseng Radix et Rhizoma were determined. The UPLC chromatographic fingerprints of chemical constituents were established from the eleven batches of the Ginseng Radix et Rhizoma and showed fifteen characteristic common peaks, among which fifteen peaks were recognized and nine compounds （ginsenosides Rgl, Re, Rf, Rg2, Rbb Rc, Rb2, Rb3 and Rd） were determined by comparison with chromatographic behaviors and UV spectra of the authentic compounds. The eleven batches of samples were classified as two clusters by hierarchical clustering analysis （HCA） and principle component analysis （PCA）, and six samples were confirmed to establish the mutual model. The quality was assessed by similarity evaluation system for chromatographic fingerprint of TCM （2004B Version）. The convenient and high specific method can be used to identify and evaluate the quality of the Ginseng Radix et Rhizoma.