川芎嗪抗大肠癌sw620裸鼠移植瘤血管生成及抑瘤机制的实验研究

Experimental study on tetramethylpyrazine in anti-angiogenesis of colorectal cancer sw620 xenografts in nude mice and its tumor-inhibitory mechanism

目的:探讨川芎嗪对大肠癌实体瘤及其血管生成的抑制作用与机制。方法:建立大肠癌sw620裸鼠移植瘤模型,随机分成5组:生理盐水组,川芎嗪低、中、高3个剂量组及恩度组。给药后检测移植瘤的体积和质量,观察移植瘤的病理形态学改变,并分别用免疫组化法和Western blotting法检测移植瘤组织中CD34、VEGF、HIF-1α蛋白表达。结果:与生理盐水组相比,川芎嗪中、高剂量组大肠癌sw620移植瘤的体积和质量明显减小,其瘤体内CD34、VEGF、HIF-1α的表达明显降低。结论:川芎嗪能抑制大肠癌sw620裸鼠移植瘤的生长,其作用机制可能与改善肿瘤组织的乏氧状况、抑制肿瘤血管生成有关。

Objective： To investigate the inhibition effects of tetramethylpyrazine on angiogenesis of solid tumor in mice and its mechanism. Methods： We established the model of colorectal cancer sw620 xenografl in nude mice and randomly divided them into five groups： NS group, low-, middle- and high-dose of tetramethylpyrazine treatment group,and Endostar group. The tumor volume and weight were measured after administration, and the pathological changes in tumors were observed . Immunohistochemistry and Western blot were used to detect the expression of CD34, VEGF, HIF-1α in tumor tissue. Results： Compared with the NS group, the size and weight of colorectal cancer sw620 xenografts in middle and high dose of tetramethylpyrazine treatment group were significantly reduced, and the expression of CD34, VEGF, HIF-1α was markedly lower. Conclusion： Tetramethylpyrazine can inhibit colorectal cancer sw620 xenograft growth in nude mice, and its mechanism may be related to the improvement of the status of tumor tissue hypoxia and to the inhibition of tumor angiogenesis.