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“接触引导”和重力场复合作用对L929细胞生长取向的影响 被引量：1

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摘要 ＂接触引导＂与外力场作用是影响细胞取向行为的两大因素.本文通过软刻蚀技术在聚二甲基硅氧烷（polydimethylsiloxane,PDMS）表面制作微米级沟槽图案,研究了当重力场方向平行于材料表面且分别垂直或平行于沟槽方向时,图案与重力的复合作用对L929细胞取向行为的影响.研究表明,当重力场方向和沟槽方向平行时,由于＂接触引导＂效应,大部分细胞（约90%）仍然沿沟槽（细胞轴向与沟槽夹角在0°~30°区间内）生长;而当重力场垂直于沟槽方向时,沿沟槽生长的细胞虽仍然占多数（约70%）,但比例显著下降.该研究表明,与重力因素相比,虽然由沟槽图案所引起的＂接触引导＂效应对细胞的生长取向起主导作用,但是当重力场与沟槽方向垂直时,重力作用显著降低了＂接触引导＂的程度;而当两种因素方向相一致时,两者对＂接触引导＂的发生无明显的协同促进作用.
作者 周峰 袁琳 梅岩 陈红
机构地区 武汉理工大学材料学院 苏州大学材料化学与化工学部
出处 《科学通报》 EI CAS CSCD 北大核心 2010年第26期2582-2586,共5页 Chinese Science Bulletin
基金 国家自然科学基金(20634030) 教育部留学回国人员启动基金(20071108)资助项目
关键词 聚二甲基硅氧烷 接触引导 重力场 细胞取向
分类号 P223 [天文地球—大地测量学与测量工程]
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参考文献12

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共引文献1

同被引文献39

  • 1曲彦隆,杨志明,谢慧琪,李绣群.三维培养状态下肌腱细胞骨架对细胞生物学行为的影响[J].中华实验外科杂志,2004,21(6):657-658. 被引量：11
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科学通报
2010年 第26期

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