Bmi-1、Ki-67在黑色素瘤中的表达及其意义

Expression and significance of Bmi-1 and Ki-67 in malignant melanoma

目的:探讨Bmi-1、Ki-67蛋白在黑色素瘤(MM)中表达及其意义。方法:采用SP免疫组化法分别检测35例黑色素瘤和14例皮内痣组织石蜡切片中Bmi-1、Ki-67蛋白的表达情况,并对Bmi-1、Ki-67行相关性分析。结果:在黑色素瘤中,Bmi-1蛋白阳性表达率与皮内痣相似(P>0.05),但中高阳性表达率明显高于皮内痣组(P<0.05);Ki-67蛋白阳性及中高阳性的表达率均较皮内痣组明显增强(P<0.01);Bmi-1蛋白中高阳性表达与病理分级密切相关(P<0.05);Ki-67蛋白中高阳性表达与患者有无淋巴结转移密切相关(P<0.05);Ki-67与Bmi-1的表达呈正相关(P<0.01)。结论:Bmi-1、Ki-67蛋白表达与黑色素瘤的发生及预后关系密切,可作为评估患者肿瘤性质及预后的参考指标。

Objective：To investigate the expression and significance of Bmi-1and Ki-67 protein in melanoma（MM）.Methods：SP immunohisto chemical technique was used to examine the expressions of Bmi-1 and Ki-67 protein in the paraffin sections of 35 cases of MM and 14 cases of intradermal nevus tissue,and to analyze the correlation of Bmi-1 and Ki-67.Results：In MM,positive expression rate of Bmi-1 was similar to that of intradermal nevi（P〈0.05）,but its high positive expression rate was obviously higher than that in nevi group（P〈0.05）.Ki-67 expression enhanced obviously compared to that of intradermal nevi group（P〈0.01）.Bmi-1 protein expression and pathologic stage was related（P〈0.05）;Ki-67 protein expression was related with lymph node metastasis（P〈0.05）;Ki-67 and Bmi-1 expression was positively correlated（P〈0.01）.Conclusion：Bmi-1,Ki-67 protein expression are related with MM,can be used as prognostic index.