65例慢性粒细胞白血病染色体核型分析及其临床意义

Analysis of karyotype in 65 cases with chronic myeloid leukemia and its clinical significance

目的:应用细胞遗传学分析方法研究65例慢性粒细胞白血病(CML)染色体异常特征及其临床意义。方法:采用短期培养法制备骨髓或外周血细胞染色体,以G显带对65例慢性粒细胞白血病患者进行染色体核型分析,并观察接受治疗后患者的临床转归。结果:65例慢性粒细胞白血病患者中,61例检出典型Ph染色体(93.85%),6例出现染色体数目异常(9.23%),3例出现复杂变异Ph易位(4.62%),4例出现附加染色体异常(6.15%),染色体数目异常者预后较差。结论:CML患者进行染色体分析对于疾病的临床治疗、预后判断具有重要意义。

Objective：To investigate the features of chromosome by cytogenetics analysis and its clinical significance in 65 cases of chronic myelogenous leukemia（CML）.Methods：Short term culture preparation bone marrow or peripheral blood cells karyotyping were performed in 65 CML patients by G-banding and to observe the patients clinical prognosis after been treated.Results：Of the 65 cases,61 cases were detected typical Ph chromosome（93.85%） and 6 patients had chromosome number abnormality（9.23%）,3 patients had variant rearrangement（4.62%）.Extra chromosome changes were demonstrated in 4 patients（6.15%）.The patients with chromosome number abnormality had worse prognosis.Conclusion：Chromosome karyotype analysis is important in the treatment and prediction of the prognosis of CML.