预针灸对大鼠血栓前状态分子标志物的影响

Experimental study on effect of pre-acupuncture and moxibustion on molecular markers in pre-thrombosis state of rats

目的:探讨防治血栓的最佳疗法及作用机制。方法:将SD大鼠40只随机分为空白组、模型组、电针组、天灸组。电针组和天灸组穴取"足三里""三阴交""脾俞""膈俞",分别用电针、天灸的方法连续干预2周后,模型组、电针组、天灸组用盐酸肾上腺素加冰水法复制血栓前状态动物模型。造模结束18h后,各组动物从腹腔静脉采血,测定大鼠血液中反映其血黏状态的分子标志物。结果:电针、天灸能使血栓前状态大鼠血液中纤溶酶原激活剂(t-PA)活性、血小板a颗粒膜蛋白140(GMP-140)含量降低,使一氧化氮(NO)含量升高。各组大鼠血液中t-PA活性、GMP-140及NO含量比较,模型组t-PA活性、GMP-140含量高于天灸组、电针组和空白组(均P<0.05),NO的含量低于天灸组、电针组和空白组,差异有统计学意义(均P<0.05);天灸组、电针组和空白组比较,差异无统计学意义(均P>0.05)。结论:电针、天灸能有效阻止血栓前状态大鼠血液中t-PA活性和GMP-140的升高,NO含量的降低;电针、天灸两种方法之间没有明显差异。

Objective To explore the best method for prevention and treatment of thrombosis and its mechanism. Methods Forty SD rats were randomly divided into a blank group, a model group, an electroacupuncture group and a crude herb moxibustion group. In the electroacupuncture group and the crude herb moxibustion group. ＂Zusanli＂ （ST 36）, ＂Sanyinjiao＂ （SP 6）, ＂Pishu＂ （BL 20） and ＂Geshu＂ （BL 17） were punctured or moxibusted for 2 weeks, then pre-thrombosis model was induced by Adrenalin Hydrochloride and ice water method in the model group, the electroacupuncture group and the crude herb moxibustion group, respectively. Molecular markers in venous blood after the model made in 18 hours were detected. Results Act.. vary of tissue-type plasmlnogen activator （t-PA） and content of alpha-granule membrane protein （GMP-140） decreased and content of nitrogen monoxidum （NO） in- creased after eleetroaeupuncture or crude herb moxihustion. The levels of t-PA and GMP-140 in the model group were higher than those in the electroacupuncture group, the crude herb moxibustion group and the blank group （all P〈0.05）, and the content of NO in the model group was lower than those in the electroacupuncture group, the crude herb moxibustion group and the blank group （all P〈0.05）, while there was no significant difference in t-PA, GMP-140 and NO among the crude herb moxibustion group, electroacupuncture group and blank group （all P〈0.05）. Conclusion Electroacupuncture and crude herb moxibustion can significantly change the contents of t- PA, GMP-140 and NO and there was no significant difference between the two therapies.