D-gal致大鼠急性肝损伤过程中肝卵圆细胞增殖及迁延

Proliferation and Protraction of Hepatic Oval Cell during Acute Liver Injury Induced by D-gal in Rat

目的用D-gal建立大鼠急性肝损伤模型,观察肝损伤后再生过程中肝卵圆细胞的增殖和迁延。方法建立大鼠急性肝损伤模型,于第1、3、7和14天分别取肝组织,分别行病理、免疫组织化学,观察卵圆细胞的分布迁移情况,并取第7天肝组织进行组织电镜观察汇管区新增生细胞超微结构。结果病理切片显示肝细胞变性坏死程度以第7天和第14天为主,出现新增生的细胞。免疫组化示随时间阳性细胞明显增多,分布于汇管区,并向小叶中心迁移,形成大量的胆小管,并有部分向坏死区迁移。透射电镜有新生内源性细胞,小于成熟的肝细胞,细胞器较少,有细胞紧密连接,以数个细胞排列成小胆管,与免疫组化一致。结论在大鼠急性肝损伤时HOC被活化、增殖,并向肝小叶中心迁移,全程参与了肝再生过程。

Objective To establish a rat model with the combination of D-galactosamine （D-GalN） ,and to observe the protraction and proliferation of oval cells during liver regeneration following hepatic damage. Methods Dgalactosamine was injected to abdominal cavity in the rat so as to duplicate liver injury model. After rats were put to death on 1,3,7 and 14 days respectively, distribution and migration of OV6 positive cells were tested by tissue immunohistochemistry and finally ultrastructure of new proliferating cells was observed by transmission electron microscope in the converge duct. Results Pathological section and immunohistochemistry results showed that HOCs gradually migrated from hepatic lobule in the portal vein to liver and confluent with normal liver cells board. OV6 positive cells were moderately increased in number by the tissue immunohistochemistry and even developed into a number of gallbladder tubules,some of which crawled into necrosis areas. Moreover,the new cells with less organells,smaller than the mature liver cells in size,lined up to form small bile duct under thetransmission electron microscope,which displayed similarities to the findings by immunohistochemistry. Conclusion HOC can be activated and proliferated following liver damage and it is involved in liver regeneration.