三种锦纶织物对红色毛癣菌和须癣毛癣菌的生长抑制效应观察

Experimental study on antifungal activity of three polyamide textiles against Trichophyton rubrum and Trichophyton mentagrophytes

目的研究三种抗真菌处理后的锦纶织物对红色毛癣菌、须癣毛癣菌生长的抑制效应,以及洗涤次数对其抗真菌作用的影响。方法制备红色毛癣菌、须癣毛癣菌的菌悬液,分别接种于洗涤1、15、30、50次的空白锦纶织物、载银1.8%锦纶织物、载银纳米氧化锌1.9%锦纶织物以及作为对照的纯棉织物,并紧贴在马铃薯葡萄糖琼脂培养基表面培养,每12h观察并记录菌落形态及大小。结果洗涤1、15、30、50次的空白锦纶织物布样上红色毛癣菌和须癣毛癣菌菌落生长情况与棉布对照组相近。载银1.8%锦纶织物洗涤1次及15次布样上菌落直径在120h内均明显小于棉布对照组(P<0.05),洗涤30及50次布样上须癣毛癣菌菌落直径在120h内与棉布对照组相近,洗涤30次布样上红色毛癣菌菌落直径在96h内小于棉布对照组(P<0.05),此后与棉布对照组相近(P>0.05)。载银纳米氧化锌1.9%锦纶织物洗涤1次的布样对两种癣菌生长的抑制作用最为明显,洗涤15次后有所降低,但两种癣菌在120h内菌落直径均小于棉布对照组(P<0.05),洗涤30次布样上红色毛癣菌菌落直径在84h内小于棉布对照组(P<0.05),之后与棉布对照组相近(P>0.05),洗涤30及50次布样上须癣毛癣菌的生长与棉布对照组相近。结论载银1.8%锦纶织物、载银纳米氧化锌1.9%锦纶织物均可显著抑制红色毛癣菌和须癣毛癣菌的生长,且具有一定的耐洗涤性,洗涤15次后仍有明显抑菌作用。

Objective To investigate the antifungal effects of three polyamide textiles treated with antifungal finishing against Trichophyton rubrum and Trichophyton mentagrophytes. Methods Three textile samples （including untreated polyamide textile as blank control, 1.8% silver-loaded polyamide textile and 1.9 % Nano-ZnO/Ag finished polyamid textile） were laid closely on the surface of potato dextrose agar following inoculation of prepared fungal suspension. Cotton textile was used as the control. Colony morphology and diameters were dynamically observed every 12hours. Results a） on blank polyamide samples, which were washed 1, 15, 30 and 50 times, the diameter of colonies of Trichophyton rubrum and Trichophyton mentagrophytes seemed to be about the same as that of cotton textile. b） Within 120h, the colonial diameters of the two species of dermatophyte under 1.8o/00 silver-loaded polyamid textiles which had been washed 1 and 15 times, were significantly smaller than that of cotton textiles （P〈0.05）. However, no significant difference was found between the colonial diameters of Trichophyton rnentagrophytes under 1. 8% silver-loaded polyamid textiles and cotton textiles after 30 and 50 cycles of washing （P〉0. 05）. The growth of Trichophyton rubrum on 30-time washed 1. 8% silver-loaded polyamid textiles was slower than that under cotton textiles within 96 hours （P〈0.05）. c） The colonial diameters of the two species of dermatophyte under 1- and 15- time washed 1. 9% Nano-ZnO/Ag finished polyamide textiles were significantly smaller than that of cotton textiles （P〈0. 05）. The growth of Trichophyton rubrum under 30- and 50-time washed 1. 9 % Nano-ZnO/Ag finished polyamid textiles was slower than that under cotton textiles within 84 hours （P〈0.05）. However, no significant difference was found between the colonial diameters of Trichophyton mentagrophytes under 1. 9% Nano-ZnO/Ag finished polyamide textiles and cotton textiles after 30 and 50 cycles of washing （P〉0. 05）. Conclusions Compared to cotton textiles, 1.8% silver-loaded polyamide textiles and 1. 9% Nano-ZnO/Ag finished polyamide textiles have antifungal effects against Trichophyton rubrum and Trichophyton mentagrophytes. Even after 15 cycles of washing, both textiles still show an effective and persistent antifungal activity.