一种新的Srrm1/SRm160可变剪接体经历无义突变介导的mRNA降解

A Novel Splicing Variant of Srrm1/ SRm160 Targets to Nonsense-Mediated mRNA Decay

目的:研究基因Srrm1/SRm160的可变剪接。方法:应用RT-PCR研究Srrm1/SRm160的可变剪接,通过蛋白质的翻译抑制和RNA干扰研究剪接异构体是否经历无义突变介导的mRNA降解(NMD)过程。结果:获得Srrm1/SRm160新的可变剪接异构体,该异构体产生提前终止密码子,翻译抑制和RNA干扰证实含有提前终止密码子的剪接体经过NMD而降解。结论:Srrm1/SRm160通过可变剪接和NMD调节自身的表达水平,作为剪接因子进一步调节其他基因的可变剪接。

Objective： To study the alternative splicing of gene Srrm1 / SRm160.Methods： RT-PCR was used to study the alternative splicing of Srrm1 / SRm160,followed by protein translation inhibition and RNA interference（RNAi） to determine if the variant is an nonsense-mediated mRNA decay（NMD） target.Results： Srrm1 / SRm160 generated a novel variant through alternative splicing.The variants contained a premature termination codon and triggerd the NMD which was confirmed by the protein translation inhibition and RNAi.Conclusion： Srrm1 / SRm160 regulates its own expression by alternative splicing and NMD,and then regulates other genes＇ alternative splicing as a splicing factor.