摘要
目的:考察摇瓶与发酵罐水平下类球红细菌CU20-9产辅酶Q10添加前体物质的最优配比。方法:通过正交试验优化发酵前体配比,考察摇瓶发酵多种前体物质的添加对辅酶Q10产量的影响,并利用发酵罐进行中试规模实验。结果:优化后发酵前体配比为维生素B1 10 mg/L、乙酸钠100 mg/L、对羟基苯甲酸20 mg/L、花生油0.01%。结论:前体添加及优化配比后,摇瓶及中试发酵水平下辅酶Q10的产量均有显著提高。
Objective： To optimize the precursors conditions of coenzyme Q10（CoQ10） produced by the Rhodobacter sphaeroides CU20-9 in shaking flask and fermenter.Methods： The precursors conditions were optimized by using the orthogonal experiments and were tested by using the B.Braun fermenter.Results： The optimum condition for the CoQ10 production were： vitamin B1 10 mg / L,sodium acetate 100 mg / L,hydroxybenzoic acid 20 mg / L,peanut oil 0.01%.Conclusion： The potency of CoQ10 was improved significantly at the optimum condition.
出处
《生物技术通讯》
CAS
2010年第5期695-698,共4页
Letters in Biotechnology
关键词
辅酶Q10
前体
正交试验
中试发酵
coenzyme Q10
precursor
orthogonal test
pilot scale fermentation
