膀胱移行细胞癌组织中PSCA和Oct-4表达及其意义

Expressions of prostate stem cell antigen and Oct-4 and their clinicopatholgical significances in transitional cell cancer of bladder

目的研究膀胱移行细胞癌和癌旁正常移行上皮组织中前列腺干细胞抗原（PSCA）和Oct-4表达水平及其临床病理意义。方法收集45例膀胱移行细胞癌和10例癌旁正常膀胱移行上皮组织标本常规制作石蜡切片,以PSCA和Oct-4标志物进行EnV ision免疫组织化学法染色。结果膀胱移行细胞癌PSCA和Oct-4表达阳性率（55.6%;53.3%）明显高于癌旁正常膀胱移行上皮组织（PSCA：10.0%,2χ=6.81,P=0.009;Oct-4：10.0%,2χ=6.20,P=0.013）。病理分级0~Ⅰ级、临床分期Tris-T1、初发病例及单发病例PSCA和Oct-4表达阳性率明显低于病理分级Ⅱ~Ⅲ级、临床分期Ⅱ~Ⅳ、复发病例及多发病例（P〈0.05或P〈0.01）。膀胱移行细胞癌中PSCA和Oct-4表达呈高度一致性（2χ=7.88,P=0.005）。结论 PSCA和Oct-4可能是反映膀胱移行细胞癌发生、进展、侵袭潜能及预后的重要生物学标志物。

Objective To study the expression levels of PSCA and Oct-4 and detect their clinicopathological significances in the peritumoral normal transitional epitheli and transitional cell cancer of bladder.Methods EnVisiom immunohistochemical method was used to assay the expression levels of PSCA and Oct-4 in the routinely paraffin-embedded sections from the resected specimens of transitional cell cancer（n=45）,peritumoral normal transitional epitheli（n=10）.Results The positive rates of PSCA and Oct-4 were higher in transitional cell cancer than those in pericancerous normal transitional epitheli（PSCA：55.6% vs 10.0%,χ2=6.81,P=0.009;Oct-4：53.3%,χ2=6.20,P=0.013）.The positive rates of PSCA and Oct-4 were significantly lower in pathological grade 0~Ⅰ,clinical stage Tris-T1,primary cases and singlet mass than those in the pathological grade Ⅱ~Ⅲ,clinical stage T2-T4,recurrent cases and multiple mass（P0.05 or P0.01）.The high consistence was found among the expressions of PSCA ans Oct-4 in transitional cell cancer（χ2=7.88,P=0.005）.Conclusion PSCA and Oct-4 might be important biological markers for reflecting the carcinogenesis,progression,invasive potential and prognosis of transitional cell cancer of bladder.