栲树微卫星分子标记的开发

Isolation of Microsatellite Markers for Castanopsis fargesii (Fagaceae)

采用基因组DNA富集法FIASCO对我国典型常绿阔叶林建群种栲树(Castanopsis fargesii Franch.)进行了微卫星分子标记的开发。从栲树基因组中分离和筛选了15个微卫星位点,并对江西九连山栲树自然分布居群的遗传多样性进行了分析。结果表明,栲树具有较高水平的遗传多样性,每个位点在28株栲树个体上的平均等位基因数(A)为6.7(4～8个),平均观察杂合度(HO)为0.690(0.250～1.000),平均预期杂合度(HE)为0.698(0.293～0.867)。每个位点的第一排除概率值Pr(Ex1)为0.043～0.527,位点综合值为0.9972。单个位点的第二排除概率Pr(EX2)为0.159～0.694,位点综合值为0.9999。这些微卫星标记可为研究栲树的遗传多样性及居群遗传结构提供有效的遗传工具。

Fifteen polymorphic microsatellite loci were developed and characterized for Castanopsis fargesii Franch. （Fagaceae）, a dominant species in typical broad-leaved evergreen forest of China. The development of microsatellite loci was conducted using the fast isolation by AFLP of sequences containing repeats protocol （FIASCO）. Fifteen microsatellite primers （SSR） were used to examine the genetic diversity of a natural population of C. fargesii in Jiulianshan Nature Reserve, Jiangxi Province. The number of alleles varied from four to eight, with a mean value of 6.7 alleles per locus. The mean observed heterozygosity （HO） and expected heterozygosity （HE） were 0.690 and 0.698, ranging from 0.250 to 1.000 and 0.293 to 0.867, respectively. The first estimate of paternity exclusion probability Pr （EX1） varied from 0.043 to 0.527 for individual loci and 0.9972 for the combined loci, when the offspring was sampled but the mother is not. The second estimate, Pr （EX2）, ranged from 0.159 to 0.694 for individual loci and 0.9999 for the combined loci, when both the mother and the offspring were sampled. It indicated that these loci could provide a powerful tool for investigating genetic diversity and population genetic structure of C. fargesii.