六月霜饮料原浆对SD大鼠致畸作用的研究

Teratogenetic effects of artemisia anomala S.Moore in SD Rats

目的:研究六月霜饮料原浆对SD大鼠母体及胚胎的毒性。方法:将交配成功的SD雌鼠随机分为4组,每组15只。设六月霜饮料原浆4.2 ml/kg、8.3 ml/kg、16.7 ml/kg体重三个剂量组及对照组,于妊娠7 d～16 d连续灌胃给药,每天1次。实验期间记录母鼠一般状况、妊娠期体重,妊娠20 d剖检母鼠,检查窝仔参数、胎仔参数及胎仔形态的变化。结果:16.7 ml/kg体重剂量组孕鼠总增重与对照组比较明显降低,差异有显著性,且胸骨6块明显减少,胸骨4块明显增加,与对照组比较差异有显著性,未见其它明显异常。结论:在本实验条件下,提示六月霜饮料原浆16.7 ml/kg体重剂量组可能有母体毒性和胚胎毒性。

Objective：To study maternal and embryo toxicity of Beverage puree of Artemisia anomala S.Moore in SD rats.Methods： SD rats fertilized successfully were divided into 4 groups（15 animals per group）.In the gestation for 7～1 6 days,animals were orally given Artemisia anomala S.Moore extract everyday at 3 doses of 4.2 ml/kg,8.3 ml/kg,16.7 ml/kg b.w..Body weight and clinical manifestations of the pregnant rats were collected during the study.On the 20th day,the pregnant rats were euthanized and examined gross anatomy,and the fetuses were removed and subsequent examinations included their count,weight,length,visceral and skeletal changes were carried out.Results：The weight increased in 16.7 ml/kg b.w.dose group was significantly lower than that in control.The quantity of fetuses with 6 pieces of sternum decreased obviously in the highest dose group,and the quantity of fetuses with 4 pieces of sternum increased obviously.No other significant difference between dose groups and control was observed.Conclusion：Beverage puree of Artemisia anomala S.Moore with 16.7 ml /kg b.w.dose group showed maternal and embryo toxicity on rats.