褪黑素改善睡眠功能实验研究

Experimental study of the Melatonin on the sleep improvement effect in mice

目的:研究褪黑素对小鼠睡眠的改善作用,并确定其产生效应较合适的剂量。方法:选用健康雌性ICR小鼠每天经口灌胃给予褪黑素,观察直接睡眠作用,连续给予受试物30d后进行延长戊巴比妥钠睡眠时间试验、戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验。结果:褪黑素剂量达到0.231 mg/kg体重时,可与巴比妥钠协同作用缩短小鼠的睡眠潜伏期;褪黑素剂量达到0.462 mg/kg和1.386 mg/kg体重时不仅能与巴比妥钠协同作用缩短小鼠的睡眠潜伏期,还能与戊巴比妥钠协同作用增加入睡小鼠的个数,具有提高入睡率的作用。结论:褪黑素能缩短小鼠的睡眠潜伏期,并能增加入睡动物个数,且以0.462 mg/kg体重的剂量较为合适。

Objective：To study the sleep improvement effect and the optimal dose of the Melatonin in mice.Methods： Healthy ICR female mice were gavaged with the melatonin everyday,and the direct sleep effect of the test material was observed.30 days later,the sleep extending test with Sodium Pentobarbital,the sleep-producing test with subthreshold dose of Sodium Pentobarbital and the incubation period test with Sodium Pentobarbital were conducted.Results： The melatonin could significantly shorten the incubation period induced by Sodium Pentobarbital in the dose of 0.231 mg/kg,0.462 mg/kg and 1.386 mg/kg B.W.,and significantly raised the numbers of the sleeping mice and the sleep rate.Conclusion： The melatonin could improve sleep of mice and its optimal dose was 0.462 mg/kg B.W.