摘要
目的:研究褪黑素对小鼠睡眠的改善作用,并确定其产生效应较合适的剂量。方法:选用健康雌性ICR小鼠每天经口灌胃给予褪黑素,观察直接睡眠作用,连续给予受试物30d后进行延长戊巴比妥钠睡眠时间试验、戊巴比妥钠阈下剂量催眠试验、巴比妥钠睡眠潜伏期试验。结果:褪黑素剂量达到0.231 mg/kg体重时,可与巴比妥钠协同作用缩短小鼠的睡眠潜伏期;褪黑素剂量达到0.462 mg/kg和1.386 mg/kg体重时不仅能与巴比妥钠协同作用缩短小鼠的睡眠潜伏期,还能与戊巴比妥钠协同作用增加入睡小鼠的个数,具有提高入睡率的作用。结论:褪黑素能缩短小鼠的睡眠潜伏期,并能增加入睡动物个数,且以0.462 mg/kg体重的剂量较为合适。
Objective:To study the sleep improvement effect and the optimal dose of the Melatonin in mice.Methods: Healthy ICR female mice were gavaged with the melatonin everyday,and the direct sleep effect of the test material was observed.30 days later,the sleep extending test with Sodium Pentobarbital,the sleep-producing test with subthreshold dose of Sodium Pentobarbital and the incubation period test with Sodium Pentobarbital were conducted.Results: The melatonin could significantly shorten the incubation period induced by Sodium Pentobarbital in the dose of 0.231 mg/kg,0.462 mg/kg and 1.386 mg/kg B.W.,and significantly raised the numbers of the sleeping mice and the sleep rate.Conclusion: The melatonin could improve sleep of mice and its optimal dose was 0.462 mg/kg B.W.
出处
《中国卫生检验杂志》
CAS
2010年第10期2482-2483,共2页
Chinese Journal of Health Laboratory Technology
关键词
褪黑素
小鼠
改善睡眠
The melatonin
Mice
Sleep improvement