特殊人群梅毒检测中检测方法的适用性分析

Study on the suitability of testing methods for syphilis examination in special populations

目的:3种梅毒检测方法比较,选择适合特殊人群梅毒感染状况的筛查方法。方法:采用ELISA法进行抗体检测,并与TRUST方法检测结果进行比较,阳性样本再用TPPA法进行确证。结果:经TPPA确认阳性的282件样本中,ELISA方法检出281件阳性,检出率为99.6%,TRUST方法检出194件阳性,检出率为68.8%,差异具显著统计学意义;ELISA方法检测样本OD值的高低与TPPA符合率有着显著差异,与TRUST符合率也有显著差异。结论:ELISA法能满足特殊人群梅毒感染状况筛查,但对OD值较低的阳性样本应再以TPPA确认。

Objective：To compare three methods of syphilis screening test,and find the most suitable one.Methods： Test serum of the samples with ELISA and TRUST,and compare the results.The positive samples are confirmed by TPPA again.Results： According to TPPA,282 samples are positive;With ELISA,the positive number is 281,and the positive rate is 99.6%.However,following TRUST,the positive samples are 194,and the rate is 68.8%.There are statistically significant difference.Compared with TPPA andTRUST,the OD values also varies in ELISA.Conclusion： ELISA is suitable for syphilis screening test in special populations.But TPPA should be used to confirm the positive samples with lower OD.