一起丧宴引致沙门菌食物中毒的微生物学检测分析

Detection and analysis of microbiology for food poisoning caused by Salmonella in the funeral dinner

目的:查找引起本次食物中毒的原因,为该起突发事件的处理提供实验依据。方法:采集引起食物中毒的相关样品共27份,依照《食品卫生微生物学检验》GB/T 4789-2008进行目的菌的分离检测;并对各分离株进行生化反应、血清学分型、药敏试验及PFGE检测;同时采集病人双份血清与分离株进行相应抗体检测。结果:在4份病人大便,1份病人肛拭,1份抹布及2份剩余食物中检出8株汤卜逊沙门菌,各分离株的生化反应、血清学分型、药敏试验结果基本一致,PFGE结果提示8株汤卜逊沙门菌有同源性;6位病人双份血清平均抗体滴度有11.28倍增长。结论:经流行病学调查和微生物学检测分析证实,这是一起因食用被汤卜逊沙门菌污染的牛肉、羊肉而引起的食物中毒事件。

Objective：To find the cause of arousing food poisoning in order to provide data for processing of the public health emergency.Methods： Purpose bacteria was isolated from twenty-seven samples of foods which were leading to the food poisoning,judging by《Microbiological Examination of Food Sanitation》GB/T 4789-2008;Each isolated strain was tested by biochemicalreaction,serotyping,drugsensitivetest and pulsed-field gel lectrophoresis,and meanwhile corresponding antibody was tested with the collecting double serum of patients and the isolated strains.Results： Eight strains of Salmonella Thompson were detected from four feces,one rectal swab specimen,one rap samples and two leftover foods of the patients,which turned out homology according to the pulsed-field gel electrophoresis.And the results of biochemicalreaction,serotyping,drugsensitivetest were similar.The geometric mean titers of double serum of six patients increased by 11.8 times.Conclusion： The food poisoning caused by Salmonella Thompson of the beef and the mutton was confirmed by the results of the detection and analysis of microbiology and the epidemiological investigation.