温州市酒店海产品中副溶血性弧菌的分型鉴定及耐药性研究

Serology identification and drug resistance analysis of Vibrio parahaemolyticus in seafood products from hotels

目的:了解温州市酒店海产品中副溶血性弧菌(Vibrio parahaemolyticusVP)污染、血清群分布及耐药状况,探讨PCR检测基因技术在鉴定中的应用,为旅游消费安全指导提供科学依据。方法:采集温州市各大酒店5类海产品共235件检测VP,以O血清分型、用PCR方法检测毒力基因(tdh、trh)及以神乃川试验检查其特定的溶血素、K-B法进行药敏试验。结果:海产品中共检出VP50株,平均检出率为21.27%。50株(其中9株已死亡)VP血清群分型属于9个血清群。37株VP经PCR结果tdh基因阳性8株;Tdhtrh阳性2株;阴性27株。50株VP神奈川试验阳性的有2株,占4.00%。挑选25株VP进行药敏试验,结果提示对CN、KZ、AK等12种药物100%敏感,对AMP、P 100%耐药;结论:酒店海产品中VP污染较为严重,药敏试验结果对青霉素类100%耐药外,其它抗生素基本敏感。PCR结果2株检出Tdh\trh阳性其神奈川试验也是阳性,神奈川试验作为毒力检测可方便于动物试验更比PCR经济实惠。血清学分型主要为O3群、O4群、O1群、O5群。

Objective：To know contamination,serogroup distribution and durg resistances about Vibrio parahaemolyticus（vp） in seafood products of hotels in wenzhou;To approach the application of PCR;To provide the scientific guidelines for the consumer of the hotel in wenzhou.Methods：A total of 235 seafood samples in hotel were collected to detest serotyping;apply PCR to test virulence（tdh,trh）;make use of Kanagawa phenomenon to test haemolysin;use K-B to test susceptibility test.Results： 50 strains of VPs were detected from 235 seafood samples.The detection rate was 21.27%.The Serology typing belong to 9 serogroups.37 strains of VPs out of 51 were tested by PCR,8 strains of VPs out of 37 is positive in tdh gene,2 strains of VPs out of 37 strains is positive in tdh/trh gene,27 strains of VPs out of 37 strains is negative.2 strains of VPs out of 51 were positive in Kanagawa phenomenon test.The detection rate was 4.00%.25 strains of VPs were picked to run medicine sensitivity tests,and they showed 100% sensitivity to 12,which are CN,KZ,AK and so on,and 100% resistance to AMP and P.Conclusion：Vibrio parahaemolyticus（vp） is more serious pollution in seafood of hotel.Medicine sensitivity test showed that VP is sensitive to generally all kinds of antibiotics except that which is 100% resistant to penicillin.2 strains of VPs is positive in the test of PCR and Kanagawa phenomenon test.Kanagawa phenomenon test can be applied to animal experiments as a method of virulence to test more economics than PCR.Serology type is mainly O3 group,O4 group,O1 group,O5 group.