高效液相色谱法测定八味肉桂胶囊中桂皮醛与桂皮酸的含量

Determination of content of cinnamic acid and cinnamaldehyde in Bawei Rougui Capsules by HPLC

目的:建立高效液相色谱法测定八味肉桂胶囊中桂皮醛与桂皮酸的含量方法。方法:色谱柱:kromasil C18柱;流动相∶乙腈-0.1%磷酸溶液(22∶78);检测波长:285nm;流速:1.0mL·min-1。结果:桂皮酸线性范围为0.0042～0.0420mg·mL-1(r=0.9999),回收率为98.28%,RSD为1.40%;桂皮醛线性范围为0.1224～1.2240mg·mL-1(r=0.9999),回收率为98.35%,RSD为1.32%。结论:本方法简便、可靠、重现性较好,能起到控制八味肉桂胶囊质量的作用。

Objective：To establish a HPLC method for the determination of cinnamaldehyde and cinnamic acid in Bawei Rougui Capsules.Methods：Cinnamic acid and cinnamaldehyde in Bawei Rougui Capsules were determined by HPLC,using kromasil Cl8 column,acetonitrile-0.1%phosphoric acid（22：78） as the mobile phase,the detection wavelength was 285nm.The flow rate was 1.0 mL·min-1.Results：The calibration curves were linear in the range of 0.0042～0.0420 mg·mL-1 for cinnamic acid （r=0.9999） and 0.1224～1.2240 mg· mL-1 for cinnamaldehyde （r=0.9999）,respectively.The average recoveries were 98.28%（RSD=1.40%） for cinnamic acid and 98.35%（RSD= 1.32%） for cinnamaldehyde.Conclusion：The method is simple,rapid,accurate and repeatable.It can be used for the quality control of Bawei Rougui Capsules.