PCR扩增16SrDNA在幽门螺杆菌感染诊断中的运用

Application of PCR Amplification of Hp 16SrDNA Fragment in the Diagnose of Hp Infection

目的 探讨16SrDNA聚合酶链式反应 （PCR）在幽门螺杆菌（Hp）感染检查中的应用价值.方法 疑似慢性胃炎、胃及十二指肠溃疡患者于胃镜下取其病变部位组织活检标本,胃癌患者于术中取病变部位组织活检标本,进行Hp的分离培养、提取活检组织DNA进行16SrDNA PCR扩增和琼脂糖凝胶电泳,并将二者结果进行比较.结果 85例病例中,54例16SrDNA PCR阳性,阳性率为63.5%;34例Hp分离培养阳性,阳性率为40%.结论 两种检测方法阳性率相比较,PCR检查法明显优于分离培养法（P＜0.005）.

Objective To investigate application value of PCR assay as a diagnostic method of gastric H. pylori （Hp） infection. Methods The gastric mucosal biopsy specimens from patients with chronic gastritis and peptic ulcer or gastric cancer were tested for Hp infection with bacteria separation and PCR amplification of Hp 16SrDNA fragment. The results of PCR amplification were compared with those of bacteria separation. Results 54 of 85（63.5%） cases were positive in PCR amplification of 16SrDNA of Hp,but 34 only were positive in Hp separation. Conclusion The positive rate of the PCR is markedly higher than that of the bacterial culture test（P〈0. 005）