胶体金法检测耐甲氧西林金黄色葡萄球菌的方法学评估

Evaluation of Dot Immunochromatography Gold Assay for Detection of Methicillin Resistant Staphylococcus Aureus

目的 评估胶体金法检测耐甲氧西林金黄色葡萄球菌的意义.方法 以胶乳凝集法作为对照,比较头孢西丁纸片扩散法、Vitek32全自动微生物鉴定/ 药敏系统和胶体金层析技术检测MRSA 的敏感度和特异度.结果 采用胶乳凝集法检测出MRSA 113株,阳性检出率为77.9%（113/145）;纸片扩散法检出MRSA 111株,阳性检出率为76.6%（111/145）;Vitek32 全自动微生物鉴定/药敏系统检出MRSA 111株,阳性检出率为76.6%（111/145）;胶体金法检出MRSA 112株,阳性检出率为77.2%（112/145）.结论 胶体金层析技术适用于临床微生物实验室.

Objective To evaluation of dot immunochromatography gold assay（DICGA） for detection of methieillin resistant Staphylococcus aureus （MRSA）. Methods The sensitivity and specificity of the method was detected MRSA by the K-B paper disk diffusion method with Cefoxitin,vitek-32 system and dot immunochromatography gold assay were com- pared with latex coagulation method. Results Of the 145 clinical isolates of Staphylococcus aureus,113 isolates showed the MRSA by latex method and the positive rate were 77.9% （113/145）;In Cefoxitin disk diffusion tests,III isolates were MRSA（76.6% ）. In Vitek32 system tests, III（76.6 % ） MRSA were detected;112（77.2% ）strains MRSA were detected by DICGA. Conclusion DICGA is reliable for routine detection of MRSA,which is suitable for clinical microbiological laboratories.