360例血小板减少病例手工复检分析及其意义

Investigation of Reexamination for 360 Thrombocytopenia Specimens with Manual Method

目的 对门诊患者中血小板减少病例的复检情况进行调查.方法 统计南京大学医学院附属鼓楼医院2009年9月～2010年1月门诊患者及健康体检者血常规检测中血小板指标低于60×109/L 的患者,涂片经瑞氏染色镜检、血小板目视计数操作进行复检,并发布最终结果.结果 期间共发现360例低于所设定界限患者,占总数的1.1%（360/34 078）.其中血常规仪器检测结果为（35±16）×109/L;目视计数结果为（44±27）×109/L.EDTA导致的血小板假性减低患者36例,占门诊及体检总例数的1‰,经目视计数结果（162±62）×109/L明显高于仪器所测结果（86±65）×109/L,二者差异具有统计学意义（P〈0.05）.结论 对于门诊血小板减少或仪器提示异常患者标本需经人工复检方能发布报告.

Objective To investigate the reexamination for thrombocytopenia specimens in outpatients. Methods To study the PLT count lower than 60×10^9/L in outpatients and the health examination bodies. The specimens were made into smears and examined in microscope ,and the sufferers of these specimens were collected peripheral blood for rechecked with manual method. Results 360 patients with a percent of 1.1% （360/34 078） were found having the results at the low side. The PLT counts were （355216）×10^9/L and （44±27）×10^9/L before and after recheck. The PLT results reexamined with manual method were different from the results analysed first time （P〈0.05） for the 36 cases of specimens with platelet aggregation. Conclusion Thrombocytopenia results in outpatients for the first time should be analysed by examining blood smears in microscope,and PLT could be reexamined with manual method or in other methods.