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不同剂量碱性成纤维细胞因子对人类胚胎干细胞生长、凋亡和DNA完整性的影响

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摘要 目的探讨碱性成纤维细胞因子(basic fibroblast growth factor,bFGF)浓度对人类胚胎干细胞(human embry-onicstem cells,hESCs)特性的影响。方法建立化学限定性hESCs培养体系,以有饲养细胞培养体系作为参照,比较4、40ng/ml和100ng/mlbFGF对hESCs多能性标记、细胞周期、细胞凋亡和DNA完整性的影响。结果①低浓度bFGF(4ng/ml)足以维持hESCs在化学限定性培养基(基础培养基DMEM/F12,N2,B27,简称NB体系)中培养;②bFGF可以抑制hESCs细胞凋亡,且具有剂量依赖性,而对hESCs细胞周期没有影响;③bFGF有助于hESCsDNA完整性维持,在NB体系加入4ng/mlbFGF时,hESCs在DNA水平上存在75个异常区域,而在加入40ng/mlbFGF时,只存在1个异常区域。结论高剂量bFGF有助于hESCs体外快速扩增和hESCsDNA完整性维持。提高bFGF浓度为hESCs体外培养所必需。
作者 罗树伟 林戈 孙争 谢平原 刘天成 卢光琇
机构地区 中南大学生殖与干细胞研究所
出处 《第三军医大学学报》 CAS CSCD 北大核心 2010年第19期2137-2139,共3页 Journal of Third Military Medical University
基金 国家自然科学基金(2006AA02A102)
关键词 人类胚胎干细胞 饲养细胞 碱性成纤维细胞生长因子 比较基因组杂交
分类号 R321 [医药卫生—人体解剖和组织胚胎学] R329.2 [医药卫生—人体解剖和组织胚胎学]
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参考文献7

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第三军医大学学报
2010年 第19期

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