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改良型TAT-VP3融合蛋白的表达、纯化及鉴定 被引量：2

Expression,Purification and Identification of Improved TAT-VP3 Fusion Protein
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摘要 目的表达改良型TAT-VP3融合蛋白,并对其进行纯化.方法利用IPTG温控诱导改良型TAT-VP3融合蛋白表达,并经GST亲和层析纯化,目的蛋白进行SDS-PAGE和Western bolt鉴定.结果改良型TAT-VP3融合蛋白经温控诱导后得到稳定表达,经SDS-PAGE鉴定可见相对分子质量42 kD的特异性目的条带,经GST亲和层析纯化后,得到了纯度较高的目的蛋白.Western bolt验证了表达蛋白的特异性.结论已成功表达及纯化出改良型TAT-VP3融合蛋白,为进一步研究其免疫原性和抗肿瘤活性奠定了基础. Objective To express and purify improved TAT-VP3 fusion protein.Methods The improved TAT-VP3 fusion protein was expressed under the induction of temperature by IPTG.The expressed product was purified by GST affinity chromatography and identified by SDS-PAGE and Western blot.Results The improved TAT-VP3 fusion protein was stably expressed under the induction of temperature.The specific band with relative molecular masses of 42000 was identified by SDS-PAGE.The expressed product was purified successfully by GST affinity chromatography and showed good specipicity as proved by Western blot.Conclusion The improved TAT-VP3 fusion protein has been successfully expressed and purified,which lay a foundation for further study on its immunogenicity and antitumor activity.
作者 张启波 王剑松 詹辉 颜汝平 杨峻峰 李俊
机构地区 昆明医学院第二附属医院泌尿外科 昆明医学院第一附属医院肾内科
出处 《昆明医学院学报》 2010年第9期4-6,26,共4页 Journal of Kunming Medical College
基金 国家自然科学基金资助项目(30760251)
关键词 改良型TAT-VP3融合蛋白 表达 纯化 鉴定 Improved TAT-VP3 fusion protein Expression Purification Identification
分类号 R996.3 [医药卫生—毒理学]
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参考文献4

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同被引文献13

  • 1王艳波,朱朝辉,曾甫清,丁小博,徐凯,张景辉.Survivin在膀胱移行细胞癌中的表达及其临床意义[J].临床泌尿外科杂志,2007,22(4):292-295. 被引量：5
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昆明医学院学报
2010年 第9期

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