Cu^(2+)离子对水丝蚓的急性毒性及超氧化物歧化酶活性的影响

Effect of Cu^(2+) on Limnodilus claparedianu Acute Toxicity and Superoxide Dismutase Activity

采用急性毒性方法,测定了不同质量分数的Cu2+离子溶液处理后的水丝蚓48h半致死浓度(LC50)为8.640μL/L;用测定酶活性方法检测了中毒后的水丝蚓的超氧化物歧化酶(SOD)活性的变化,与对照组比较,Cu2+离子对动物SOD活性影响显著(P<0.05)。低浓度药物(0.500~5.340)μL/L处理动物,SOD活性呈上升趋势;当浓度增加至11.740μL/L时,SOD活性开始表现为不同程度的降低。说明Cu2+离子对水丝蚓造成胁迫,直至动物个体死亡,间接或直接地影响水生生态系统及以水丝蚓为食物的下一级消费者。

The author adopted an acute toxicity method to mensure the semi-lethal concentration （LC 50 ） of Limnodilus claparedianus that were disposed in Cu 2 ＋ with different concentrations. The variation of L. claparedianus SOD activity toxic was tested by means of enzyme activity in this paper. The results showed that the semi-lethal concentration （LC 50 ） of 48 hours was 8.640 μ L/L. Cu 2＋ had a significant effect on L. hoffmeisteri SOD activity （P〈0.05）. The SOD activity in the animal specimen increased when they were disposed in low concentration Cu 2 ＋ （0.500~5.340 μ L/L）. But SOD activity in the animal specimen decreased at different levels when concentrations of Cu 2 ＋ increased to 11.740 μ L/L, a conclusion was achieved that Cu 2 ＋ could cause coercion to L. claparedianus and even cause death of animal individuals, and they could also lead to direct and indirect influence on aquatic ecosystems and the subspecies which were fed by L. claparedianus.