NF-κB在急性坏死性胰腺炎相关性肺损伤中的表达改变及乌斯他丁的治疗作用

Protective effect of ulinastatin on acute pancreatitis associated lung injuries in rats

目的:研究NF-κB在急性坏死性胰腺炎(ANP)相关性肺损伤中的表达改变情况及乌斯他丁的治疗作用机制。方法:采用逆行胰胆管牛磺胆酸钠(TAC)注射方法建立大鼠ANP肺损伤模型。SD大鼠分为假手术组(SO组),ANP生理盐水对照组(ANP组)和乌斯他丁治疗组。各组于术后3,6,12h剖杀,检验NF-κB的表达情况及肺组织髓过氧化物酶(MPO)活性和TNF-α的变化及肺组织的病理学改变。结果:相对于SO组,ANP组大鼠从3h开始,MPO及TNF-α增高,NF-κB表达于3h增高,6h达到高峰,给予乌斯他丁治疗后,肺损伤各指标均有不同程度降低(P<0.05),NF-κB表达明显降低(P<0.05)。结论:ANP相关肺损伤与NF-κB的激活有关,乌斯他丁通过下调NF-κB表达对ANP相关性肺损伤起保护作用。

Objective：To investigate the change of NF-κB expression and the protective effect of ulinastatin（UST）in acute lung injury of acute necrotizing pancreatitis（ANP）.Method：Murine modle of ANP was established by retrograde injection of artificial bile into the biliopancreatic duct.The Sprague-Dawley were divided into sham operation group（SO group）,ANP saline injection group（ANP group）,and ANP UST treated group（UST group）.Each group were sacrificed at 3,6,12 h after the operation to test the expression of NF-κB,myeloperoxidase（MPO）activity in lung tissue,the change of TNF-α,and the pathological changes of lung tissue.Result：Compared with sham operated group,TNF-a,MOP level of ANP group in lung tissie were increased from 3 h,and NF-κB was significantly increased at 3 h,peaking at 6 h.In contrast,TNF-αand MOP level were markedly decreased（P0.05）and the expression of NF-κB was inhibited（P0.05）after treated by UST.Conclusion：The expression of NF-κB is inceasd in lung in ANP,UST had an protective effect in lung injuries in ANP by down-regulating NF-κB.