糖尿病微血管病SUMO基因163 A/G多态性的相关性研究

Association of SUMO gene 163 A/G polymorphism with diabetic microangiopathy

目的:探讨小泛素样修饰蛋白(SUMO)基因163A/G多态性与糖尿病微血管病变相关性。方法:运用聚合酶链反应-限制性片段长度多态性方法,对30例2型糖尿病(T2DM)患者[正常白蛋白尿(DM0)组10例,微量白蛋白尿(DM1)组10例,大量白蛋白尿(DM2)组10例]和10例健康对照者(NC)的SUMO基因163A/G多态性进行检测,并比较各组间基因型频率、等位基因频率及相关临床指标。结果:本组实验人群中SUMO基因163位点存在3种基因型,即AA型、GA型和GG型。NC组与T2DM组各基因型和等位基因频率无显著差异(x2=2.231,P=0.121;x2=0.015,P=0.906);DM组(DM1+DM2)的GA基因型频率高于DM0组(x2=4.454,P=0.038);DM1组与DM2组基因型和等位基因频率无统计学差异(x2=0.041,P=0.973;x2=0.123,P=0.683)。Logistic回归分析显示SUMO基因GA基因型(OR=2.31,P<0.05)与糖尿病肾病相关。结论:SUMO基因GA基因型可能是糖尿病微血管病发生的危险因素。

Objective： To investigate the association of small ubiquitin related modifier protein （SUMO） gene 163 A/G polymorphism with diabetic microangiopathy. Methods： The polymorphism of SUMO gene 163 A/G was detected by polymerase chain reaction-restriction fragment length polymorphism （PCR--RFLP） method in 30 patients with type 2 diabetes mellitus （T2DM）, including 10 cases with normal albuminuria （DM0 group）, 10 cases with mieroalbuminuria （DM1 group）, 10 cases with macroalbuminuria （DM2 group） and 10 normal controls （NC group） . The genotype frequencies, allele frequencies and clinic indexes were compared among all groups. Results： There were three genotype, i.e. AA genetype, GA genetype and GG genetype of SUM（） gene 163 sites. Except the frequency of GA genetype in DM group （DMI＋ DM2） was higher than that in DM0 group （X2=41.454. P 0.038）, there was no difference in genetype frequencies and allele frequencies between NC group and T2DM group （x2=2.231, P=0.121; x2=0.015, P：=0.906） and betweenDMlgroup and DM2 group （x2=0.041- P=0.973; x2=0.123, P=0.683）. Logistic re- gression analysis show that GA genetype of SUMO gene was related with diabetic nephropathy （OR=2. 131, P〈 0.05）. Conclusion：. The GA genetype of SLIMO gene may be associated with diabetic microangiopatby.